Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5363264..5363889 Replicon chromosome
Accession NZ_CP101563
Organism Klebsiella pneumoniae subsp. pneumoniae strain KP65

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NMY31_RS26450 Protein ID WP_002882817.1
Coordinates 5363264..5363647 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NMY31_RS26455 Protein ID WP_004150355.1
Coordinates 5363647..5363889 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMY31_RS26435 (NMY31_26415) 5360630..5361532 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NMY31_RS26440 (NMY31_26420) 5361529..5362164 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NMY31_RS26445 (NMY31_26425) 5362161..5363090 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NMY31_RS26450 (NMY31_26430) 5363264..5363647 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NMY31_RS26455 (NMY31_26435) 5363647..5363889 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NMY31_RS26460 (NMY31_26440) 5364094..5365011 + 918 WP_002882812.1 alpha/beta hydrolase -
NMY31_RS26465 (NMY31_26445) 5365025..5365966 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NMY31_RS26470 (NMY31_26450) 5366011..5366448 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NMY31_RS26475 (NMY31_26455) 5366445..5367305 - 861 WP_255846630.1 virulence factor BrkB family protein -
NMY31_RS26480 (NMY31_26460) 5367299..5367898 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T252312 WP_002882817.1 NZ_CP101563:c5363647-5363264 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT252312 WP_004150355.1 NZ_CP101563:c5363889-5363647 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References