Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5444355..5444980 Replicon chromosome
Accession NZ_CP101554
Organism Klebsiella pneumoniae subsp. pneumoniae strain KP72

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NMY33_RS26840 Protein ID WP_002882817.1
Coordinates 5444355..5444738 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NMY33_RS26845 Protein ID WP_004150355.1
Coordinates 5444738..5444980 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMY33_RS26825 (NMY33_26815) 5441721..5442623 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NMY33_RS26830 (NMY33_26820) 5442620..5443255 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NMY33_RS26835 (NMY33_26825) 5443252..5444181 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NMY33_RS26840 (NMY33_26830) 5444355..5444738 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NMY33_RS26845 (NMY33_26835) 5444738..5444980 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NMY33_RS26850 (NMY33_26840) 5445185..5446102 + 918 WP_004187175.1 alpha/beta hydrolase -
NMY33_RS26855 (NMY33_26845) 5446116..5447057 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NMY33_RS26860 (NMY33_26850) 5447102..5447539 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NMY33_RS26865 (NMY33_26855) 5447536..5448396 - 861 WP_019725422.1 virulence factor BrkB family protein -
NMY33_RS26870 (NMY33_26860) 5448390..5448989 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T252291 WP_002882817.1 NZ_CP101554:c5444738-5444355 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT252291 WP_004150355.1 NZ_CP101554:c5444980-5444738 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References