Detailed information of TA system TA025229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1975339..1975970
• Replicon: chromosome
• Accession: NC_015520
• Organism: Mahella australiensis 50-1 BON

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F4A1A6
• Locus tag: MAHAU_RS09205
• Protein ID: WP_013781453.1
• Coordinates: 1975339..1975689 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: F4A1A7
• Locus tag: MAHAU_RS09210
• Protein ID: WP_013781454.1
• Coordinates: 1975698..1975970 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAHAU_RS09180	1970399..1971076	-	678	WP_013781449.1	hypothetical protein	-
MAHAU_RS09185	1971073..1972287	-	1215	WP_013781450.1	hypothetical protein	-
MAHAU_RS09190	1972256..1973116	-	861	WP_013781451.1	diadenylate cyclase CdaA	-
MAHAU_RS09200	1973326..1975149	-	1824	WP_013781452.1	heme NO-binding domain-containing protein	-
MAHAU_RS09205	1975339..1975689	-	351	WP_013781453.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MAHAU_RS09210	1975698..1975970	-	273	WP_013781454.1	ribbon-helix-helix protein, CopG family	Antitoxin
MAHAU_RS09215	1976046..1977593	-	1548	WP_013781455.1	bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase	-
MAHAU_RS09220	1977605..1978069	-	465	WP_013781456.1	chemotaxis protein CheX	-
MAHAU_RS09225	1978639..1979106	-	468	WP_013781457.1	SsrA-binding protein SmpB	-
MAHAU_RS09230	1979208..1980683	-	1476	WP_013781458.1	stage IV sporulation protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12753.87 Da
• Isoelectric Point: 8.4124

>T25229 WP_013781453.1 NC_015520:c1975689-1975339 [Mahella australiensis 50-1 BON]
MIVKRGDLFYADLSPVVGSEQGGVRPVLVVQNDIGNKYSPTVIVAAITSQINKAKLPTHVEISASDYGLPKDSVILLEQI
RTIDKKRLREKIGYLSAETMKKVDEALQISFGLIEL

Nucleotide

• Length: 351 bp

>T25229 NC_015520:c1975689-1975339 [Mahella australiensis 50-1 BON]
ATGATTGTAAAGCGAGGAGATTTGTTTTATGCCGATCTAAGCCCTGTGGTTGGTTCCGAGCAGGGCGGAGTTCGGCCGGT
GTTGGTAGTGCAGAACGATATAGGTAATAAATATAGCCCTACTGTCATAGTAGCAGCCATAACATCGCAGATCAATAAAG
CGAAATTGCCCACGCATGTGGAAATAAGCGCTTCTGATTACGGTTTACCTAAAGATTCCGTAATACTCTTAGAACAAATA
CGTACCATAGACAAAAAACGCTTAAGAGAAAAAATAGGCTATCTTTCGGCTGAGACTATGAAAAAAGTGGACGAAGCATT
ACAGATAAGCTTTGGCCTTATAGAATTATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10432.10 Da
• Isoelectric Point: 4.9336

>AT25229 WP_013781454.1 NC_015520:c1975970-1975698 [Mahella australiensis 50-1 BON]
MSVSKKILVSLPDSLLKEVDEIVAMEKKNRSEFIREAMKLYIRERQKIEIKEQMIKGYQDMARINEDMAEIGVKADNYAL
NAYESFIGGE

Nucleotide

• Length: 273 bp

>AT25229 NC_015520:c1975970-1975698 [Mahella australiensis 50-1 BON]
ATGTCGGTTTCCAAAAAGATACTAGTTAGCCTGCCCGATAGTCTATTGAAAGAGGTAGACGAGATTGTGGCTATGGAGAA
GAAGAACAGAAGTGAATTTATAAGAGAAGCTATGAAGCTTTATATAAGAGAAAGGCAGAAGATAGAGATAAAAGAGCAAA
TGATTAAGGGTTATCAGGATATGGCCAGGATAAATGAGGATATGGCCGAGATCGGTGTCAAGGCCGACAATTATGCCCTT
AACGCGTATGAAAGCTTTATTGGCGGAGAGTGA

Structures

Toxin

• AlphaFold DB: F4A1A6

Antitoxin

• AlphaFold DB: F4A1A7