Detailed information of TA system TA025228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 2525772..2526322
• Replicon: chromosome
• Accession: NC_015519
• Organism: Tepidanaerobacter acetatoxydans Re1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F4LTT6
• Locus tag: TEPRE1_RS12135
• Protein ID: WP_013779452.1
• Coordinates: 2526047..2526322 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F4LTT5
• Locus tag: TEPRE1_RS12130
• Protein ID: WP_013779451.1
• Coordinates: 2525772..2526050 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TEPRE1_RS12100	2521775..2522248	-	474	WP_013779446.1	phage terminase small subunit P27 family	-
TEPRE1_RS12105	2522288..2522581	-	294	WP_013779447.1	hypothetical protein	-
TEPRE1_RS12110	2522799..2522987	-	189	WP_015295921.1	hypothetical protein	-
TEPRE1_RS12115	2522980..2523741	-	762	WP_013779448.1	hypothetical protein	-
TEPRE1_RS12120	2523828..2524127	-	300	WP_013779449.1	hypothetical protein	-
TEPRE1_RS12125	2524237..2525469	-	1233	WP_041591602.1	DNA modification methylase	-
TEPRE1_RS12130	2525772..2526050	+	279	WP_013779451.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
TEPRE1_RS12135	2526047..2526322	+	276	WP_013779452.1	type II toxin-antitoxin system YafQ family toxin	Toxin
TEPRE1_RS12140	2526352..2526738	-	387	WP_013779453.1	HNH endonuclease	-
TEPRE1_RS12145	2526701..2526937	-	237	WP_013779454.1	hypothetical protein	-
TEPRE1_RS12155	2527183..2527614	-	432	WP_013779455.1	DUF1492 domain-containing protein	-
TEPRE1_RS12160	2527604..2527819	-	216	WP_013779456.1	hypothetical protein	-
TEPRE1_RS12165	2527822..2529177	-	1356	WP_013779457.1	DEAD/DEAH box helicase	-
TEPRE1_RS12170	2529174..2529452	-	279	WP_013779458.1	VRR-NUC domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2480652..2537225	56573

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-79)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10775.47 Da
• Isoelectric Point: 7.8830

>T25228 WP_013779452.1 NC_015519:2526047-2526322 [Tepidanaerobacter acetatoxydans Re1]
MSRQILWTTQFKKDYKLAEKRGLNITLLDDCIRMLATGEKLPPEFRDHNLSGRWSGYRECHIQPDWLLIYRIDVDNLILV
LVRTGSHSDLF

Nucleotide

• Length: 276 bp

>T25228 NC_015519:2526047-2526322 [Tepidanaerobacter acetatoxydans Re1]
ATGAGCCGCCAGATTTTGTGGACAACACAATTTAAAAAGGATTATAAGCTTGCGGAAAAACGTGGACTTAATATTACCCT
ATTAGATGATTGTATCCGCATGCTTGCAACCGGAGAAAAGTTGCCACCCGAATTCCGTGACCATAACCTTTCCGGCAGAT
GGAGTGGTTATCGTGAGTGCCATATTCAGCCTGACTGGCTTTTGATTTACCGTATTGATGTTGATAATTTAATTCTTGTT
TTAGTTCGAACCGGTAGCCATAGCGATTTGTTTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10205.59 Da
• Isoelectric Point: 4.4367

>AT25228 WP_013779451.1 NC_015519:2525772-2526050 [Tepidanaerobacter acetatoxydans Re1]
MAKKTASVSFRIDADIKKQADELFAELGLNMTTAFNIFLRQSIREGRIPFDVTLNTPNAVTVAALLEAEQIANNSDAKPY
FDVEEALQELKS

Nucleotide

• Length: 279 bp

>AT25228 NC_015519:2525772-2526050 [Tepidanaerobacter acetatoxydans Re1]
TTGGCCAAAAAAACAGCTAGTGTTAGCTTTAGAATCGATGCCGATATTAAAAAACAGGCGGATGAACTGTTTGCAGAGCT
TGGACTTAATATGACTACAGCCTTCAATATTTTTCTACGCCAATCAATAAGAGAAGGACGTATTCCCTTTGATGTCACTC
TAAATACCCCTAATGCTGTGACAGTGGCCGCTCTACTTGAAGCAGAGCAAATTGCTAATAACTCAGATGCTAAACCTTAT
TTTGATGTCGAGGAAGCGCTGCAGGAGTTAAAATCATGA

Structures

Toxin

• AlphaFold DB: F4LTT6

Antitoxin

• AlphaFold DB: F4LTT5