Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5172399..5173024 Replicon chromosome
Accession NZ_CP101546
Organism Klebsiella pneumoniae subsp. pneumoniae strain KP82

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag NMY34_RS25540 Protein ID WP_002882817.1
Coordinates 5172399..5172782 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag NMY34_RS25545 Protein ID WP_004150355.1
Coordinates 5172782..5173024 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMY34_RS25525 (NMY34_25510) 5169765..5170667 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
NMY34_RS25530 (NMY34_25515) 5170664..5171299 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
NMY34_RS25535 (NMY34_25520) 5171296..5172225 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
NMY34_RS25540 (NMY34_25525) 5172399..5172782 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
NMY34_RS25545 (NMY34_25530) 5172782..5173024 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
NMY34_RS25550 (NMY34_25535) 5173229..5174146 + 918 WP_002882812.1 alpha/beta hydrolase -
NMY34_RS25555 (NMY34_25540) 5174160..5175101 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
NMY34_RS25560 (NMY34_25545) 5175146..5175583 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
NMY34_RS25565 (NMY34_25550) 5175580..5176440 - 861 WP_002882807.1 virulence factor BrkB family protein -
NMY34_RS25570 (NMY34_25555) 5176434..5177033 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T252273 WP_002882817.1 NZ_CP101546:c5172782-5172399 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT252273 WP_004150355.1 NZ_CP101546:c5173024-5172782 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References