Detailed information of TA system TA025227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1574452..1575052
• Replicon: chromosome
• Accession: NC_015518
• Organism: Acidianus hospitalis W1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F4B6S1
• Locus tag: AHOS_RS09060
• Protein ID: WP_013776529.1
• Coordinates: 1574452..1574838 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F4B6S2
• Locus tag: AHOS_RS09065
• Protein ID: WP_013776530.1
• Coordinates: 1574828..1575052 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AHOS_RS13355 (Ahos_1734)	1569936..1570463	+	528	WP_013776526.1	hypothetical protein	-
AHOS_RS09040	1570464..1570667	+	204	WP_048054723.1	NB-ARC domain-containing protein	-
AHOS_RS09045 (Ahos_1735)	1570678..1571766	+	1089	WP_013776527.1	hypothetical protein	-
AHOS_RS09050	1571963..1572468	-	506	Protein_1813	PaREP1 family protein	-
AHOS_RS09055 (Ahos_1736)	1572957..1574132	+	1176	WP_013776528.1	cytosine permease	-
AHOS_RS09060 (Ahos_1737)	1574452..1574838	-	387	WP_013776529.1	type II toxin-antitoxin system VapC family toxin	Toxin
AHOS_RS09065 (Ahos_1738)	1574828..1575052	-	225	WP_013776530.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
AHOS_RS09070	1575248..1575970	+	723	Protein_1817	IS5/IS1182 family transposase	-
AHOS_RS09075 (Ahos_1740)	1576675..1577538	-	864	WP_013776532.1	CRISPR system precrRNA processing endoribonuclease RAMP protein Cas6	-
AHOS_RS09080 (Ahos_1741)	1577709..1578692	-	984	WP_013776533.1	type I-A CRISPR-associated protein CsaX	-
AHOS_RS09085 (Ahos_1742)	1578673..1579380	-	708	WP_048054954.1	CRISPR-associated endonuclease Cas3''	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1575472..1575699	227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (7-119)

Antitoxin

• Post-segregation antitoxin CcdA (6-49)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14701.21 Da
• Isoelectric Point: 8.2953

>T25227 WP_013776529.1 NC_015518:c1574838-1574452 [Acidianus hospitalis W1]
MKGEFLLDSSALYPLLNYADKVDVSKIYILSLTFYEVGNAIWKEFYIHKRIKDPFTLSLLFQKFLHKLKVLSDPPANEVM
KVAVDKGLTFYDASYVYSAEANSLTLVSEDKELIKKANAIPLKEFIRL

Nucleotide

• Length: 387 bp

>T25227 NC_015518:c1574838-1574452 [Acidianus hospitalis W1]
ATGAAAGGTGAGTTTCTTTTAGATTCTTCAGCTTTATATCCCCTTCTAAATTACGCCGATAAAGTAGACGTAAGTAAAAT
TTACATTCTTTCCCTCACCTTTTACGAGGTAGGTAATGCCATATGGAAGGAATTTTATATTCACAAGAGGATTAAAGATC
CTTTCACTCTCTCTTTACTATTTCAAAAGTTCTTACATAAGCTCAAAGTTTTAAGCGACCCTCCTGCTAACGAGGTTATG
AAGGTTGCTGTAGATAAGGGACTAACTTTTTACGACGCGTCATATGTTTACTCCGCCGAAGCTAATAGCCTTACCTTAGT
CTCAGAGGATAAGGAGTTAATAAAGAAAGCCAACGCCATTCCGCTAAAGGAATTTATTAGATTGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8806.11 Da
• Isoelectric Point: 4.7127

>AT25227 WP_013776530.1 NC_015518:c1575052-1574828 [Acidianus hospitalis W1]
MSVVLSVRVRKELKEKAEQLGINIRDVVEKALEEAIKEKEKEEINDIARKIKELMKDVSEEEWVEAIREERNER

Nucleotide

• Length: 225 bp

>AT25227 NC_015518:c1575052-1574828 [Acidianus hospitalis W1]
ATGTCAGTCGTATTGAGCGTAAGAGTCAGGAAGGAACTTAAGGAGAAGGCTGAGCAACTAGGAATAAACATTAGGGATGT
TGTTGAGAAGGCTCTTGAGGAGGCAATTAAGGAGAAGGAAAAGGAGGAGATTAATGATATTGCTAGGAAGATCAAGGAGT
TGATGAAGGACGTTAGTGAGGAAGAGTGGGTTGAAGCAATAAGGGAGGAGAGGAATGAAAGGTGA

Structures

Toxin

• AlphaFold DB: F4B6S1

Antitoxin

• AlphaFold DB: F4B6S2