Detailed information of TA system TA025224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 410089..410734
• Replicon: chromosome
• Accession: NC_015518
• Organism: Acidianus hospitalis W1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F4B5T6
• Locus tag: AHOS_RS02125
• Protein ID: WP_013775217.1
• Coordinates: 410345..410734 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F4B5T5
• Locus tag: AHOS_RS02120
• Protein ID: WP_013775216.1
• Coordinates: 410089..410358 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AHOS_RS02090 (Ahos_0407)	405110..405694	+	585	WP_013775211.1	hypothetical protein	-
AHOS_RS02095 (Ahos_0408)	405807..406835	-	1029	WP_013775212.1	phospholipase D-like domain-containing protein	-
AHOS_RS12365	407393..408537	+	1145	Protein_431	RNA-guided endonuclease TnpB family protein	-
AHOS_RS02115 (Ahos_0411)	408675..409856	-	1182	WP_013775215.1	MFS transporter	-
AHOS_RS02120 (Ahos_0412)	410089..410358	+	270	WP_013775216.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AHOS_RS02125 (Ahos_0413)	410345..410734	+	390	WP_013775217.1	type II toxin-antitoxin system VapC family toxin	Toxin
AHOS_RS02130 (Ahos_0414)	410775..411398	-	624	WP_013775218.1	type 1 glutamine amidotransferase	-
AHOS_RS02135 (Ahos_0415)	411452..411814	-	363	WP_013775219.1	GntR family transcriptional regulator	-
AHOS_RS13185 (Ahos_0416)	411951..413120	-	1170	WP_013775220.1	hypothetical protein	-
AHOS_RS12915	413120..413266	-	147	WP_187287287.1	hypothetical protein	-
AHOS_RS02145 (Ahos_0417)	413330..414514	+	1185	WP_013775221.1	carboxypeptidase CpsA	-
AHOS_RS02150 (Ahos_0418)	414542..414958	-	417	Protein_440	amidohydrolase family protein	-
AHOS_RS02155	415307..415552	+	246	WP_048054507.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• SpoVT-AbrB domain (4-46)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 15197.75 Da
• Isoelectric Point: 4.7591

>T25224 WP_013775217.1 NC_015518:410345-410734 [Acidianus hospitalis W1]
MIFLDANFIIYLNLGVKEVVNFYIKVLQEDRLALDPLVIDEVIYISKKKYNVNFNDTISFLDEVVLPNSLILPIRKEDYD
KAKEIMLQYNLKPSDAFHVAIMLNNSISKIISEDKNFDRIKEIERLWLN

Nucleotide

• Length: 390 bp

>T25224 NC_015518:410345-410734 [Acidianus hospitalis W1]
ATGATTTTTCTTGATGCAAACTTTATCATATACTTGAATTTGGGTGTCAAAGAAGTAGTAAATTTTTATATTAAAGTATT
ACAAGAAGATCGATTAGCTTTAGACCCGTTAGTTATAGATGAAGTAATTTATATTTCAAAGAAGAAATATAACGTTAACT
TTAACGATACAATAAGTTTTCTAGATGAAGTTGTTTTACCCAATTCTCTAATCCTTCCTATAAGAAAAGAAGATTATGAC
AAGGCAAAAGAAATAATGCTACAATATAACCTAAAGCCCTCTGACGCGTTTCACGTCGCCATAATGTTAAATAACTCAAT
CAGTAAAATTATAAGTGAAGATAAGAATTTTGATCGAATTAAAGAAATAGAGAGACTTTGGTTAAATTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10202.86 Da
• Isoelectric Point: 5.7585

>AT25224 WP_013775216.1 NC_015518:410089-410358 [Acidianus hospitalis W1]
MTLKVEVGKKGYIIIPKDIRDLLGIKEGDKLILNVYDGKIILEPERKVEINEILKRLEEHHAKISSYAKSAKLGDLLYSS
LEEEFNDFS

Nucleotide

• Length: 270 bp

>AT25224 NC_015518:410089-410358 [Acidianus hospitalis W1]
TTGACTCTAAAGGTGGAAGTTGGAAAGAAAGGTTATATAATTATTCCTAAGGATATTAGAGATCTTTTAGGCATAAAAGA
AGGTGACAAGCTTATTCTTAACGTCTATGACGGTAAAATTATCCTTGAACCTGAAAGGAAGGTTGAAATTAATGAGATCT
TAAAAAGGCTGGAAGAGCATCATGCTAAAATATCCTCTTATGCAAAAAGCGCTAAATTAGGAGACTTACTTTATTCAAGC
CTTGAGGAGGAATTCAATGATTTTTCTTGA

Structures

Toxin

• AlphaFold DB: F4B5T6

Antitoxin

• AlphaFold DB: F4B5T5