Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Xre(antitoxin)
Location 4450910..4452168 Replicon chromosome
Accession NZ_CP101540
Organism Pseudomonas aeruginosa strain D-2

Toxin (Protein)


Gene name - Uniprot ID Q9I4U4
Locus tag NNL25_RS20655 Protein ID WP_003112528.1
Coordinates 4450910..4451665 (-) Length 252 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID V6AJU7
Locus tag NNL25_RS20660 Protein ID WP_003086329.1
Coordinates 4451800..4452168 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NNL25_RS20645 (NNL25_20615) 4446082..4448625 + 2544 WP_033876574.1 acyl-homoserine-lactone acylase QuiP -
NNL25_RS20650 (NNL25_20620) 4448780..4450141 - 1362 WP_003081943.1 DNA recombination protein RmuC -
NNL25_RS20655 (NNL25_20625) 4450910..4451665 - 756 WP_003112528.1 RES family NAD+ phosphorylase Toxin
NNL25_RS20660 (NNL25_20630) 4451800..4452168 - 369 WP_003086329.1 DUF2384 domain-containing protein Antitoxin
NNL25_RS20665 (NNL25_20635) 4452241..4453527 - 1287 WP_255865287.1 FAD-binding oxidoreductase -
NNL25_RS20670 (NNL25_20640) 4453583..4455172 - 1590 WP_003125055.1 aldehyde dehydrogenase family protein -
NNL25_RS20675 (NNL25_20645) 4455251..4455727 + 477 WP_003112531.1 hypothetical protein -
NNL25_RS20680 (NNL25_20650) 4455730..4456005 - 276 WP_169057979.1 zinc ribbon domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(63-207)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-47)


Sequences

Toxin        


Download         Length: 252 a.a.        Molecular weight: 28278.42 Da        Isoelectric Point: 7.7294

>T252233 WP_003112528.1 NZ_CP101540:c4451665-4450910 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Download         Length: 756 bp

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13367.63 Da        Isoelectric Point: 9.9874

>AT252233 WP_003086329.1 NZ_CP101540:c4452168-4451800 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Download         Length: 369 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I4U4

Antitoxin

Source ID Structure
AlphaFold DB V6AJU7

References