Detailed information of TA system TA025223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/NGN_SP-AbrB
• Location: 398923..399520
• Replicon: chromosome
• Accession: NC_015518
• Organism: Acidianus hospitalis W1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F4B5S3
• Locus tag: AHOS_RS02050
• Protein ID: WP_013775204.1
• Coordinates: 399137..399520 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AHOS_RS02045
• Protein ID: WP_187287286.1
• Coordinates: 398923..399153 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AHOS_RS02020 (Ahos_0394)	394529..394762	+	234	WP_013775198.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
AHOS_RS02025 (Ahos_0395)	394755..395141	+	387	WP_048054503.1	type II toxin-antitoxin system VapC family toxin	-
AHOS_RS02030 (Ahos_0396)	395359..396684	-	1326	WP_013775200.1	IS200/IS605 family accessory protein TnpB-related protein	-
AHOS_RS02035	396656..397226	-	571	Protein_418	IS607 family transposase	-
AHOS_RS02040 (Ahos_0398)	397411..398661	-	1251	WP_013775202.1	MFS transporter	-
AHOS_RS02045 (Ahos_0399)	398923..399153	+	231	WP_187287286.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AHOS_RS02050 (Ahos_0400)	399137..399520	+	384	WP_013775204.1	type II toxin-antitoxin system VapC family toxin	Toxin
AHOS_RS02055 (Ahos_0401)	399653..399889	+	237	WP_013775205.1	hypothetical protein	-
AHOS_RS02060 (Ahos_0402)	399871..401535	+	1665	WP_013775206.1	DNA polymerase domain-containing protein	-
AHOS_RS02065 (Ahos_0403)	401532..402098	+	567	WP_013775207.1	hypothetical protein	-
AHOS_RS02070 (Ahos_0404)	402171..402683	-	513	WP_013775208.1	hypothetical protein	-
AHOS_RS02075 (Ahos_0405)	402828..404117	-	1290	WP_013775209.1	RNA-guided endonuclease TnpB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	391601..408537	16936

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-117)

Antitoxin

• SpoVT-AbrB domain (5-45)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14356.69 Da
• Isoelectric Point: 9.4984

>T25223 WP_013775204.1 NC_015518:399137-399520 [Acidianus hospitalis W1]
VKVVLDASTLILYFYGNNKAKEIIEKNMNEAFINSVNLTEFLYSYAKVKGWKESLFKYTLIRDTFKLIDVNSEDIIKSVA
KLKIKYNLSLGDAFLLASAKSTNSTAVTSDHELSKVNEVKVLLIPKE

Nucleotide

• Length: 384 bp

>T25223 NC_015518:399137-399520 [Acidianus hospitalis W1]
GTGAAAGTAGTACTTGACGCCTCTACATTAATTTTGTATTTTTATGGAAATAACAAGGCCAAAGAGATAATAGAGAAAAA
CATGAATGAAGCTTTTATAAATTCGGTAAATCTTACAGAATTCCTTTATTCATATGCTAAAGTCAAAGGCTGGAAGGAAT
CTCTATTTAAATATACCTTGATCAGGGATACATTTAAGCTAATAGATGTTAACAGCGAGGACATAATTAAAAGCGTGGCC
AAGTTGAAAATTAAGTATAACCTCTCCTTAGGAGACGCGTTCTTGCTAGCATCTGCCAAAAGTACAAATTCAACTGCCGT
AACAAGTGATCACGAGCTTAGTAAAGTTAATGAGGTTAAAGTACTGCTTATTCCTAAGGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 9011.31 Da
• Isoelectric Point: 5.9050

>AT25223 WP_187287286.1 NC_015518:398923-399153 [Acidianus hospitalis W1]
MYTLKVHKKGIIVIPKEIREKFNLKEGDEVNVIVDDEGIYIFPRDDIRKYFGSDKDAIKALNILEEERRRERESST

Nucleotide

• Length: 231 bp

>AT25223 NC_015518:398923-399153 [Acidianus hospitalis W1]
ATGTATACACTGAAGGTTCATAAGAAAGGTATTATTGTAATACCAAAGGAGATAAGAGAAAAATTCAACTTGAAAGAAGG
TGACGAAGTTAACGTTATAGTTGATGATGAGGGAATTTATATATTTCCTCGTGATGATATTAGAAAATACTTTGGTAGTG
ACAAAGATGCAATAAAAGCTTTAAACATTCTGGAAGAGGAGAGGAGAAGAGAACGTGAAAGTAGTACTTGA

Structures

Toxin

• AlphaFold DB: F4B5S3