Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2562764..2563415 Replicon chromosome
Accession NZ_CP101538
Organism Lacticaseibacillus rhamnosus strain HN067

Toxin (Protein)


Gene name pemK Uniprot ID K8Q4Y0
Locus tag NNM43_RS11915 Protein ID WP_005686631.1
Coordinates 2562764..2563147 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K8QH19
Locus tag NNM43_RS11920 Protein ID WP_005686632.1
Coordinates 2563167..2563415 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NNM43_RS11910 (NNM43_11950) 2561800..2562327 - 528 WP_005686630.1 QueT transporter family protein -
NNM43_RS11915 (NNM43_11955) 2562764..2563147 - 384 WP_005686631.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
NNM43_RS11920 (NNM43_11960) 2563167..2563415 - 249 WP_005686632.1 antitoxin Antitoxin
NNM43_RS11925 (NNM43_11965) 2563527..2564666 - 1140 WP_005686633.1 alanine racemase -
NNM43_RS11930 (NNM43_11970) 2564653..2565027 - 375 WP_005686634.1 holo-ACP synthase -
NNM43_RS11935 (NNM43_11975) 2565196..2566704 - 1509 WP_005686635.1 DEAD/DEAH box helicase -
NNM43_RS11940 (NNM43_11980) 2566978..2568366 - 1389 WP_005686636.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13757.03 Da        Isoelectric Point: 10.9764

>T252227 WP_005686631.1 NZ_CP101538:c2563147-2562764 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8936.13 Da        Isoelectric Point: 4.4778

>AT252227 WP_005686632.1 NZ_CP101538:c2563415-2563167 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K8Q4Y0

Antitoxin

Source ID Structure
AlphaFold DB K8QH19

References