Detailed information of TA system TA025219

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 168585..169150
• Replicon: chromosome
• Accession: NC_015500
• Organism: Treponema brennaborense DSM 12168

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: TREBR_RS00705
• Protein ID: WP_041610495.1
• Coordinates: 168585..168908 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: F4LLC2
• Locus tag: TREBR_RS00710
• Protein ID: WP_013757320.1
• Coordinates: 168902..169150 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TREBR_RS00685	164198..165094	+	897	WP_013757315.1	LysR family transcriptional regulator	-
TREBR_RS00690	165191..166279	+	1089	WP_013757316.1	S1 RNA-binding domain-containing protein	-
TREBR_RS00695	166509..167342	+	834	WP_013757317.1	AraC family transcriptional regulator	-
TREBR_RS14205	167749..167913	-	165	WP_169310616.1	hypothetical protein	-
TREBR_RS00700	168041..168508	-	468	WP_013757318.1	DUF3795 domain-containing protein	-
TREBR_RS00705	168585..168908	-	324	WP_041610495.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TREBR_RS00710	168902..169150	-	249	WP_013757320.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TREBR_RS00715	169366..170496	+	1131	WP_013757258.1	IS110 family transposase	-
TREBR_RS00720	170766..171509	-	744	WP_013757321.1	NERD domain-containing protein	-
TREBR_RS00725	171510..171962	-	453	WP_013757322.1	hypothetical protein	-
TREBR_RS13990	172260..172481	-	222	WP_013757323.1	DUF1697 domain-containing protein	-
TREBR_RS00730	172524..172817	-	294	WP_083816409.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• SpoVT-AbrB domain (8-47)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12372.76 Da
• Isoelectric Point: 9.9840

>T25219 WP_041610495.1 NC_015500:c168908-168585 [Treponema brennaborense DSM 12168]
MVVNQYEVFLIDLDPTMGHDMQKTRPCLVLSPNEMNHHISTVIVAPMTTKSHSFPTRVQLKFAEKQGWIVLDQIRTIDKV
RLVKKVGKIETKTIKKVKEVLMEMLID

Nucleotide

• Length: 324 bp

>T25219 NC_015500:c168908-168585 [Treponema brennaborense DSM 12168]
ATGGTAGTTAATCAATATGAAGTATTTCTCATTGATTTAGACCCAACGATGGGACATGATATGCAGAAAACAAGACCTTG
TTTAGTGCTCTCGCCAAATGAAATGAATCATCATATTAGTACAGTAATCGTTGCACCTATGACAACGAAATCACACAGTT
TTCCAACGCGAGTACAATTGAAATTTGCAGAAAAGCAAGGGTGGATTGTGTTGGATCAAATACGAACAATAGACAAGGTT
CGCTTAGTGAAGAAGGTGGGAAAGATTGAAACAAAAACAATAAAGAAAGTGAAAGAAGTCTTGATGGAAATGCTCATTGA
TTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9419.86 Da
• Isoelectric Point: 4.6128

>AT25219 WP_013757320.1 NC_015500:c169150-168902 [Treponema brennaborense DSM 12168]
MLVSLVVIGNSKGIRLPKTVIDQLQVKDKLELEIEDSKIVLKPVKSKARQGWNESFINMHQTNEDTIIDEAPIESEAFEW
VW

Nucleotide

• Length: 249 bp

>AT25219 NC_015500:c169150-168902 [Treponema brennaborense DSM 12168]
ATGCTAGTGTCGCTTGTCGTAATAGGTAATTCCAAAGGAATAAGACTGCCCAAAACAGTCATAGATCAACTACAAGTAAA
GGATAAGCTCGAATTAGAAATAGAAGACAGCAAGATTGTGCTTAAACCAGTAAAGTCAAAAGCTCGGCAAGGCTGGAATG
AATCATTTATCAACATGCATCAAACAAATGAGGATACTATCATTGATGAGGCTCCGATTGAATCCGAGGCATTTGAATGG
GTATGGTAG

Structures

Antitoxin

• AlphaFold DB: F4LLC2