Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 85938..86736 Replicon plasmid pHH194M-140K
Accession NZ_CP101514
Organism Escherichia coli strain HH194M

Toxin (Protein)


Gene name ataT Uniprot ID -
Locus tag MOO21_RS23110 Protein ID WP_072662620.1
Coordinates 86215..86736 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A141BRA2
Locus tag MOO21_RS23105 Protein ID WP_001711191.1
Coordinates 85938..86207 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOO21_RS23070 (MOO21_23065) 81111..81293 + 183 Protein_91 iron-containing alcohol dehydrogenase -
MOO21_RS23075 (MOO21_23070) 81448..82182 - 735 WP_000578338.1 helix-turn-helix domain-containing protein -
MOO21_RS23080 (MOO21_23075) 82272..82454 + 183 WP_232580417.1 PerC family transcriptional regulator -
MOO21_RS23090 (MOO21_23085) 83774..84541 + 768 WP_001711193.1 hypothetical protein -
MOO21_RS23095 (MOO21_23090) 84594..84947 - 354 WP_161953766.1 hypothetical protein -
MOO21_RS23100 (MOO21_23095) 84953..85618 - 666 WP_023135658.1 AAA family ATPase -
MOO21_RS23105 (MOO21_23100) 85938..86207 + 270 WP_001711191.1 DUF1778 domain-containing protein Antitoxin
MOO21_RS23110 (MOO21_23105) 86215..86736 + 522 WP_072662620.1 GNAT family N-acetyltransferase Toxin
MOO21_RS23115 (MOO21_23110) 86960..87268 - 309 Protein_100 integrase core domain-containing protein -
MOO21_RS23120 (MOO21_23115) 87285..88361 - 1077 WP_038989268.1 IS110-like element ISEc21 family transposase -
MOO21_RS23125 (MOO21_23120) 88643..89239 - 597 Protein_102 IS3 family transposase -
MOO21_RS23130 (MOO21_23125) 89227..89307 + 81 Protein_103 hypothetical protein -
MOO21_RS23135 (MOO21_23130) 89304..90446 + 1143 Protein_104 IS30 family transposase -
MOO21_RS23140 (MOO21_23135) 90459..90716 - 258 Protein_105 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / tet(A) / blaTEM-1B - 1..140492 140492
- inside IScluster/Tn - - 79263..90716 11453


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(48-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19359.18 Da        Isoelectric Point: 8.2462

>T252181 WP_072662620.1 NZ_CP101514:86215-86736 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLTNHLKRQHEGKILRAYVLCTKEEIPKVLGYYTLSGSCFEKETLPSKSQQ
KKVPYRNVPSITLGRLALDKSLQGQGLGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKARAFYKSLGFIQLVGNNDRSL
FYPTKSIEKLFEE

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10054.53 Da        Isoelectric Point: 8.7353

>AT252181 WP_001711191.1 NZ_CP101514:85938-86207 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLSEESWNLVMDAISNPPAPNDKLKR
AANRLKSME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A141BRA2

References