Detailed information of TA system TA252180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 72612..73213
• Replicon: plasmid pHH194M-140K
• Accession: NZ_CP101514
• Organism: Escherichia coli strain HH194M

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: MOO21_RS23005
• Protein ID: WP_001216039.1
• Coordinates: 72612..72992 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: MOO21_RS23010
• Protein ID: WP_001190711.1
• Coordinates: 72992..73213 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOO21_RS22975 (MOO21_22970)	67853..68557	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
MOO21_RS22980 (MOO21_22975)	68621..69082	+	462	Protein_73	recombinase family protein	-
MOO21_RS22985 (MOO21_22980)	69265..70125	+	861	WP_000027057.1	broad-spectrum class A beta-lactamase TEM-1	-
MOO21_RS22990 (MOO21_22985)	70272..70831	-	560	Protein_75	IS1 family transposase	-
MOO21_RS22995 (MOO21_22990)	70892..71500	+	609	Protein_76	PAS domain-containing protein	-
MOO21_RS23000 (MOO21_22995)	71663..72376	+	714	WP_224965575.1	methyl-accepting chemotaxis protein	-
MOO21_RS23005 (MOO21_23000)	72612..72992	-	381	WP_001216039.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
MOO21_RS23010 (MOO21_23005)	72992..73213	-	222	WP_001190711.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MOO21_RS23015 (MOO21_23010)	73478..74175	+	698	WP_225907799.1	IS1 family transposase	-
MOO21_RS23020 (MOO21_23015)	74192..74518	-	327	Protein_81	hypothetical protein	-
MOO21_RS23025 (MOO21_23020)	74591..75853	-	1263	WP_069067570.1	hypothetical protein	-
MOO21_RS23030 (MOO21_23025)	76155..76856	-	702	WP_104730539.1	hypothetical protein	-
MOO21_RS23035 (MOO21_23030)	76853..77530	-	678	WP_104730538.1	metallophosphoesterase	-
MOO21_RS23040 (MOO21_23035)	77527..78153	-	627	WP_000484116.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / tet(A) / blaTEM-1B	-	1..140492	140492
	-	inside	IScluster/Tn	blaTEM-1B	-	66209..74175	7966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13558.27 Da
• Isoelectric Point: 5.1514

>T252180 WP_001216039.1 NZ_CP101514:c72992-72612 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRAALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T252180 NZ_CP101514:c72992-72612 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTGATAGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTGCCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8134.08 Da
• Isoelectric Point: 4.5843

>AT252180 WP_001190711.1 NZ_CP101514:c73213-72992 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVDR

Nucleotide

• Length: 222 bp

>AT252180 NZ_CP101514:c73213-72992 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCTCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTGACCGATAA