Detailed information of TA system TA025212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH
• Location: 1725168..1725846
• Replicon: chromosome
• Accession: NC_015460
• Organism: Gallibacterium anatis UMN179

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F4HBW4
• Locus tag: UMN179_RS08035
• Protein ID: WP_013746373.1
• Coordinates: 1725463..1725846 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: UMN179_RS08030
• Protein ID: WP_013746372.1
• Coordinates: 1725168..1725470 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
UMN179_RS08005	1720421..1721404	+	984	WP_013746367.1	DUF979 domain-containing protein	-
UMN179_RS08010	1721414..1722406	+	993	WP_013746368.1	DUF2891 domain-containing protein	-
UMN179_RS08015	1722817..1723533	+	717	WP_039096894.1	amino acid ABC transporter permease	-
UMN179_RS08020	1723536..1724279	+	744	WP_026211038.1	amino acid ABC transporter ATP-binding protein	-
UMN179_RS08025	1724827..1725072	+	246	WP_013746371.1	hypothetical protein	-
UMN179_RS08030	1725168..1725470	-	303	WP_013746372.1	DNA-binding transcriptional regulator	Antitoxin
UMN179_RS08035	1725463..1725846	-	384	WP_013746373.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
UMN179_RS08040	1726046..1726324	+	279	WP_013746374.1	type II toxin-antitoxin system RelE/ParE family toxin	-
UMN179_RS08045	1726335..1726631	+	297	WP_013746375.1	HigA family addiction module antidote protein	-
UMN179_RS08050	1726662..1727249	-	588	Protein_1556	Fic family protein	-
UMN179_RS08055	1727218..1727409	-	192	WP_013746378.1	antitoxin VbhA family protein	-
UMN179_RS08060	1728091..1729470	-	1380	WP_013746380.1	cysteine--tRNA ligase	-
UMN179_RS08065	1729610..1730122	+	513	WP_013746381.1	peptidylprolyl isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-120)

Antitoxin

• Cro/C1-type helix-turn-helix domain (45-88)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14213.20 Da
• Isoelectric Point: 8.9256

>T25212 WP_013746373.1 NC_015460:c1725846-1725463 [Gallibacterium anatis UMN179]
MRIFKTKAFNKFALKNGIPDSELINAITRAEQGLIDADLGGNIIKQRIARKGQGRSGGFRSFIFYCIQNNSYFVAGISKN
DRENISSQELAALKALAREYTRLTAKQIEAQIENGLFIEILPEAEDE

Nucleotide

• Length: 384 bp

>T25212 NC_015460:c1725846-1725463 [Gallibacterium anatis UMN179]
ATGAGAATATTTAAAACTAAAGCTTTTAACAAATTTGCTTTAAAAAATGGTATTCCTGACAGCGAATTAATCAATGCTAT
AACGAGGGCAGAGCAAGGATTAATAGATGCTGATTTAGGCGGTAATATTATTAAGCAGAGAATAGCCAGAAAAGGGCAGG
GACGTAGTGGTGGATTCCGTTCTTTTATTTTTTATTGTATCCAAAATAACAGTTATTTTGTTGCCGGAATTAGTAAAAAT
GACAGAGAAAATATTTCATCACAAGAGTTAGCAGCGTTAAAGGCATTGGCTAGAGAATATACTCGACTAACAGCAAAACA
GATTGAAGCACAGATCGAAAACGGATTATTCATCGAAATTTTACCGGAGGCAGAGGATGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11270.16 Da
• Isoelectric Point: 7.3581

>AT25212 WP_013746372.1 NC_015460:c1725470-1725168 [Gallibacterium anatis UMN179]
MSELLAMIHENAADLHKAGIINKKTMKEFDQLCLTPVVTYTPDEIRAIREKEQVSQAVFAHYLNVSKNMISEWERGVKKP
SGAALKLLNLVQHNGIEILV

Nucleotide

• Length: 303 bp

>AT25212 NC_015460:c1725470-1725168 [Gallibacterium anatis UMN179]
ATGAGTGAATTGTTGGCAATGATCCACGAAAATGCGGCGGATTTACATAAAGCCGGCATTATTAATAAAAAAACAATGAA
AGAATTTGATCAGCTTTGTTTGACACCTGTTGTAACTTATACGCCGGACGAAATTAGAGCGATTCGTGAAAAAGAACAGG
TATCCCAAGCGGTATTCGCACATTATCTTAATGTGAGTAAAAATATGATTTCCGAATGGGAACGAGGCGTTAAAAAACCA
AGTGGTGCAGCATTAAAGTTGCTTAATTTGGTACAGCATAATGGTATTGAGATTCTTGTGTAG

Structures

Toxin

• AlphaFold DB: F4HBW4