Detailed information of TA system TA025209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1562531..1563127
• Replicon: chromosome
• Accession: NC_015458
• Organism: Pusillimonas sp. T7-7

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F4GVF3
• Locus tag: PT7_RS07000
• Protein ID: WP_013742513.1
• Coordinates: 1562789..1563127 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: F4GVF2
• Locus tag: PT7_RS06995
• Protein ID: WP_013742512.1
• Coordinates: 1562531..1562761 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PT7_RS06985	1559005..1559685	+	681	WP_013742510.1	epoxyqueuosine reductase QueH	-
PT7_RS06990	1559775..1562369	-	2595	WP_013742511.1	ATP-dependent chaperone ClpB	-
PT7_RS06995	1562531..1562761	+	231	WP_013742512.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
PT7_RS07000	1562789..1563127	+	339	WP_013742513.1	endoribonuclease MazF	Toxin
PT7_RS07005	1563312..1564040	+	729	WP_041682611.1	helix-turn-helix domain-containing protein	-
PT7_RS07010	1564093..1564347	-	255	WP_041682612.1	membrane protein	-
PT7_RS07015	1564377..1564589	-	213	WP_041683103.1	FixH family protein	-
PT7_RS07020	1564626..1566125	-	1500	WP_013742517.1	cytochrome c oxidase accessory protein CcoG	-
PT7_RS07025	1566136..1567059	-	924	WP_013742518.1	cytochrome-c oxidase, cbb3-type subunit III	-
PT7_RS07030	1567052..1567225	-	174	WP_013742519.1	cytochrome oxidase	-
PT7_RS07035	1567226..1567882	-	657	WP_013742520.1	cytochrome-c oxidase, cbb3-type subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-108)

Antitoxin

• SpoVT-AbrB domain (6-50)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12337.18 Da
• Isoelectric Point: 8.4957

>T25209 WP_013742513.1 NC_015458:1562789-1563127 [Pusillimonas sp. T7-7]
MNKRYTPDTGDIIHLHFDLPGSSTESSYKPALVLSPMAYNKKTGLVVCCAITNDIKGYPFEVQVQSEQPLAVLSDQVKSI
DWAHTRISQHGRVNAAELAQVRAKLLALISKT

Nucleotide

• Length: 339 bp

>T25209 NC_015458:1562789-1563127 [Pusillimonas sp. T7-7]
ATGAACAAGCGCTATACGCCAGATACCGGCGACATCATCCACCTGCACTTCGACCTGCCTGGCAGCAGTACCGAAAGCAG
CTATAAGCCCGCATTGGTGCTCAGCCCCATGGCCTACAATAAAAAAACCGGGCTTGTGGTCTGTTGCGCCATCACAAACG
ATATCAAGGGCTACCCATTTGAAGTTCAAGTTCAATCCGAGCAGCCTCTGGCCGTGCTGTCGGACCAGGTCAAAAGCATA
GATTGGGCCCATACCCGGATCAGCCAGCATGGCCGAGTCAACGCCGCCGAACTAGCACAAGTGCGCGCCAAGCTGCTGGC
CCTGATTTCCAAGACCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8371.79 Da
• Isoelectric Point: 9.8654

>AT25209 WP_013742512.1 NC_015458:1562531-1562761 [Pusillimonas sp. T7-7]
MRGVIKKWGNSAAIRLPSSMLKSLKLSADDIVELRLERGTIVIQPVRSEEYLLGQLLSGIKPKNIHPEIDFGAASD

Nucleotide

• Length: 231 bp

>AT25209 NC_015458:1562531-1562761 [Pusillimonas sp. T7-7]
ATGCGCGGTGTAATCAAAAAATGGGGTAATAGCGCTGCGATACGCCTGCCCTCATCCATGCTGAAATCGCTGAAGCTCAG
CGCTGACGACATCGTGGAACTTCGGCTCGAGCGCGGCACGATAGTGATACAGCCCGTACGCTCCGAAGAATATCTGCTTG
GGCAATTGTTAAGCGGCATCAAGCCAAAGAACATACATCCTGAAATAGATTTTGGCGCAGCCAGCGACTAA

Structures

Toxin

• AlphaFold DB: F4GVF3

Antitoxin

• AlphaFold DB: F4GVF2