Detailed information of TA system TA025201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/HTH_10(toxin)
• Location: 976873..977849
• Replicon: chromosome
• Accession: NC_015435
• Organism: Metallosphaera cuprina Ar-4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MCUP_RS05220
• Protein ID: WP_048057506.1
• Coordinates: 977235..977849 (+)
• Length: 205 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MCUP_RS05215
• Protein ID: WP_048057737.1
• Coordinates: 976873..977217 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MCUP_RS05205 (Mcup_1063)	972206..974527	+	2322	WP_013737666.1	protein kinase	-
MCUP_RS05210 (Mcup_1065)	974630..976438	+	1809	WP_148230906.1	NosD domain-containing protein	-
MCUP_RS05215 (Mcup_1066)	976873..977217	+	345	WP_048057737.1	DUF488 domain-containing protein	Antitoxin
MCUP_RS05220 (Mcup_1067)	977235..977849	+	615	WP_048057506.1	helix-turn-helix domain-containing protein	Toxin
MCUP_RS05225 (Mcup_1068)	978360..978788	+	429	WP_013737671.1	hypothetical protein	-
MCUP_RS05230 (Mcup_1069)	979431..979802	+	372	WP_013737672.1	hypothetical protein	-
MCUP_RS05235	979975..980190	-	216	WP_148230907.1	hypothetical protein	-
MCUP_RS05240 (Mcup_1070)	980372..981547	-	1176	WP_013737673.1	FAD/NAD(P)-binding oxidoreductase	-
MCUP_RS05245 (Mcup_1071)	981759..982361	-	603	WP_013737674.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterioopsin activator-type, HTH domain (145-194)

Antitoxin

• Protein of unknown function DUF488 (3-108)

Sequences

Toxin

Protein

• Length: 205 a.a.
• Molecular weight: 23518.42 Da
• Isoelectric Point: 9.7734

>T25201 WP_048057506.1 NC_015435:977235-977849 [Metallosphaera cuprina Ar-4]
VRKTPFEVTVLIEDHPCEAMRVISQTGMKASVENVKLREQTTDHVVVFEKEIEKNDILKLKSNNLKVLRLTDRKVWVRTN
GCAVCKTLYASDVVVEKVKVIKERNLLYTLLVPNNSSLKELLYNLSQNKLKVTVLNVSEISDTELTERQLEILKLAYKLG
YFNDDRKITLTQLAEKLGVSAPTLEEILRRASRKSVKYYLDRHT

Nucleotide

• Length: 615 bp

>T25201 NC_015435:977235-977849 [Metallosphaera cuprina Ar-4]
GTGCGTAAGACACCCTTTGAGGTCACAGTTCTAATAGAAGATCATCCTTGTGAAGCAATGAGGGTGATCTCGCAAACCGG
TATGAAGGCCAGCGTTGAGAACGTTAAGTTGAGAGAACAAACGACGGATCACGTAGTCGTTTTTGAAAAAGAGATTGAGA
AGAACGATATATTGAAATTAAAGTCTAATAATCTCAAAGTATTAAGACTAACCGATAGAAAGGTTTGGGTAAGAACTAAC
GGTTGCGCGGTCTGTAAGACACTTTACGCCTCTGATGTCGTCGTGGAGAAGGTTAAAGTGATTAAGGAGAGGAACTTGTT
GTACACCTTGCTAGTACCTAATAACTCCTCTTTGAAGGAATTGTTGTATAACCTTTCACAGAATAAGTTGAAGGTAACGG
TCCTGAACGTTTCAGAGATATCAGATACAGAATTAACTGAAAGACAATTAGAAATATTAAAATTGGCATATAAATTAGGA
TACTTTAATGATGATAGGAAGATAACTCTAACTCAGTTGGCGGAGAAGTTAGGCGTTTCCGCTCCGACTTTAGAGGAGAT
ATTGCGTAGGGCCTCAAGAAAATCAGTTAAATATTATTTGGACAGACACACGTAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13624.82 Da
• Isoelectric Point: 10.0963

>AT25201 WP_048057737.1 NC_015435:976873-977217 [Metallosphaera cuprina Ar-4]
VIKLKRVYEPVDKDDGIRVLVDRLWPRGVSKSKVDVWLKDVAPSEDLRRWFNHDPSKWEEFKRRYFDELDKNPKIEILLQ
LIRKNTNVTLVYASKSPYNNAVALKEYLEKLLKP

Nucleotide

• Length: 345 bp

>AT25201 NC_015435:976873-977217 [Metallosphaera cuprina Ar-4]
GTGATAAAGCTGAAGAGGGTTTACGAACCCGTCGATAAGGATGATGGGATAAGGGTACTAGTGGACAGGCTATGGCCCAG
AGGCGTTAGTAAGAGTAAGGTTGACGTGTGGCTTAAAGACGTTGCTCCCAGTGAGGACTTGAGGAGGTGGTTCAACCACG
ATCCAAGCAAATGGGAGGAGTTTAAGAGGAGGTACTTTGACGAGTTGGATAAAAATCCTAAAATTGAGATACTATTACAA
TTAATCAGGAAAAATACTAACGTGACTTTGGTCTACGCCTCTAAATCACCTTACAACAACGCAGTTGCATTAAAAGAATA
CCTTGAGAAGCTATTAAAACCGTGA