Detailed information of TA system TA251980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4169301..4169877
• Replicon: chromosome
• Accession: NZ_CP101373
• Organism: Salmonella enterica strain SC2016042

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: NMU32_RS20485
• Protein ID: WP_001131963.1
• Coordinates: 4169590..4169877 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: NMU32_RS20480
• Protein ID: WP_000063142.1
• Coordinates: 4169301..4169603 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NMU32_RS20465 (4165811)	4165811..4167961	+	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-
NMU32_RS20470 (4168056)	4168056..4168259	+	204	WP_000460616.1	YbdD/YjiX family protein	-
NMU32_RS20475 (4168270)	4168270..4169226	+	957	WP_000187839.1	GTPase	-
NMU32_RS20480 (4169301)	4169301..4169603	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
NMU32_RS20485 (4169590)	4169590..4169877	-	288	WP_001131963.1	BrnT family toxin	Toxin
NMU32_RS20490 (4170146)	4170146..4171060	-	915	WP_000290512.1	restriction endonuclease	-
NMU32_RS20495 (4171258)	4171258..4174767	+	3510	WP_001043484.1	type I restriction-modification system endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T251980 WP_001131963.1 NZ_CP101373:c4169877-4169590 [Salmonella enterica]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T251980 NZ_CP101373:c4169877-4169590 [Salmonella enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT251980 WP_000063142.1 NZ_CP101373:c4169603-4169301 [Salmonella enterica]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT251980 NZ_CP101373:c4169603-4169301 [Salmonella enterica]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01