Detailed information of TA system TA025194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 4504874..4506393
• Replicon: chromosome
• Accession: NC_015422
• Organism: Alicycliphilus denitrificans K601

Toxin (Protein)

• Gene name: HipA1
• Uniprot ID: F4G7M0
• Locus tag: ALIDE2_RS21345
• Protein ID: WP_013723103.1
• Coordinates: 4504874..4506151 (-)
• Length: 426 a.a.

Antitoxin (Protein)

• Gene name: HipB1
• Uniprot ID: -
• Locus tag: ALIDE2_RS24730
• Protein ID: WP_013723104.1
• Coordinates: 4506148..4506393 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALIDE2_RS21325	4500552..4501802	-	1251	WP_013520729.1	FIST C-terminal domain-containing protein	-
ALIDE2_RS21330	4501827..4502609	-	783	WP_013520730.1	hypothetical protein	-
ALIDE2_RS21335	4502837..4503268	+	432	WP_013520731.1	HU family DNA-binding protein	-
ALIDE2_RS21340	4503326..4504078	-	753	WP_013520732.1	tyrosine-protein phosphatase	-
ALIDE2_RS24725	4504615..4504905	-	291	WP_202945487.1	hypothetical protein	-
ALIDE2_RS21345	4504874..4506151	-	1278	WP_013723103.1	type II toxin-antitoxin system HipA family toxin	Toxin
ALIDE2_RS24730	4506148..4506393	-	246	WP_013723104.1	helix-turn-helix transcriptional regulator	Antitoxin
ALIDE2_RS24735	4506596..4506790	-	195	WP_193353132.1	hypothetical protein	-
ALIDE2_RS21350	4506780..4507817	-	1038	Protein_4276	IS630 family transposase	-
ALIDE2_RS21355	4508028..4509158	+	1131	WP_013721273.1	IS630 family transposase	-
ALIDE2_RS21360	4509205..4509417	+	213	WP_148262990.1	hypothetical protein	-
ALIDE2_RS21365	4509693..4510907	-	1215	WP_085945338.1	IS4 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-102)

Antitoxin

• Transcription regulator, HipB-like (10-66)

Sequences

Toxin

Protein

• Length: 426 a.a.
• Molecular weight: 46609.57 Da
• Isoelectric Point: 7.0792

>T25194 WP_013723103.1 NC_015422:c4506151-4504874 [Alicycliphilus denitrificans K601]
MSRVLEAWLLGQHVGQLAQVDGRLSFCYSTQWLQSANARPLSHHLPLQAEAFDDKATRPFFAGLLPEGDKRRLIAQTLHV
SRQNDFALLDGIGGECAGAVSLLEPGQRPDLIASAQAVRWLDEAELIALLDELPLRPMLAGEEGLRLSLAGAQDKLPVVF
AEGRVGLPLQNTPSSHILKPTIQAVEGSVFNEGFCMALAAQMKLTAASAAIHRVADRVYLLVDRYDRVRQPGGALLRLHQ
EDFCQALGIAPEYKYQNEGGPDLAQCFALIRKTTRPSAPHILRLLDYVIFNALIGNHDAHAKNFSLIYTGRGTVLAPLYD
ALSTAVYDNLTDKMAMKIGSKYKFTEVQVRHWEQFAQAAGLSPAQVKKRILALARQLPSEAASLRSLFATRELDHDILGE
VVGLIERRCQLTCKRLEAAGPGGDE

Nucleotide

• Length: 1278 bp

>T25194 NC_015422:c4506151-4504874 [Alicycliphilus denitrificans K601]
ATGAGCCGCGTCCTGGAAGCCTGGCTGCTGGGGCAGCACGTCGGACAGCTTGCACAAGTCGATGGTCGGCTGAGTTTTTG
CTACTCAACGCAATGGCTGCAATCCGCCAACGCACGTCCACTGTCGCATCACCTCCCCTTGCAGGCGGAGGCTTTTGACG
ACAAGGCCACGCGCCCCTTCTTTGCCGGCCTTCTGCCTGAAGGCGATAAGCGCAGACTGATTGCCCAGACCTTGCACGTC
TCGCGCCAGAACGACTTTGCCCTGCTCGATGGCATCGGCGGTGAATGCGCTGGGGCGGTCAGTCTGCTAGAACCTGGCCA
GCGGCCCGATCTCATCGCCTCGGCGCAGGCCGTGCGATGGTTGGACGAGGCAGAACTGATCGCGCTCCTGGATGAACTGC
CCCTCCGCCCCATGTTGGCTGGGGAGGAAGGCTTGCGCCTGTCACTGGCGGGGGCGCAGGACAAGCTGCCCGTTGTCTTT
GCCGAGGGACGCGTCGGCCTGCCTCTGCAGAACACCCCAAGTTCCCACATTCTCAAGCCAACCATTCAGGCTGTAGAAGG
CAGCGTGTTCAATGAGGGCTTCTGCATGGCACTGGCCGCGCAGATGAAGCTGACTGCCGCCAGCGCAGCCATCCATCGCG
TGGCTGATCGCGTGTATTTGCTGGTGGACCGATATGACCGCGTGCGCCAACCCGGAGGTGCCTTGCTGCGGCTGCACCAG
GAGGACTTCTGCCAAGCATTGGGTATTGCACCCGAGTACAAGTACCAAAACGAGGGCGGCCCGGACCTGGCCCAATGCTT
CGCGCTGATACGCAAAACCACACGCCCAAGCGCTCCGCACATTTTGCGCTTACTGGACTACGTGATCTTCAACGCCCTGA
TTGGCAACCACGATGCGCACGCCAAGAACTTCTCCCTGATCTACACCGGACGCGGTACGGTGTTGGCACCGCTCTACGAC
GCGCTATCGACAGCGGTCTACGACAACCTCACCGACAAGATGGCGATGAAAATCGGCAGCAAGTACAAATTCACCGAGGT
TCAAGTGCGGCATTGGGAACAGTTTGCACAGGCTGCTGGGCTGTCACCCGCACAGGTCAAAAAAAGGATCCTGGCTCTGG
CGCGCCAGTTGCCCTCAGAAGCGGCCTCCCTGCGTTCTTTGTTTGCCACACGGGAATTGGATCACGACATACTCGGTGAG
GTCGTAGGTCTCATCGAACGGCGTTGCCAACTCACCTGCAAGCGGCTTGAAGCCGCAGGGCCTGGTGGTGATGAATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8626.96 Da
• Isoelectric Point: 7.2031

>AT25194 WP_013723104.1 NC_015422:c4506393-4506148 [Alicycliphilus denitrificans K601]
MPTLIETPEHLGDVVRHTRKALSLTQPQLALAAGVGVRFIVDLEAGKPTVRLENILRVLQVLGATLSVSSSEDSQTQAGP
K

Nucleotide

• Length: 246 bp

>AT25194 NC_015422:c4506393-4506148 [Alicycliphilus denitrificans K601]
ATGCCTACACTGATTGAAACGCCAGAGCACTTGGGCGACGTGGTGCGCCATACCCGAAAAGCACTCAGCCTGACCCAGCC
CCAACTAGCGCTTGCAGCCGGCGTGGGGGTGCGGTTTATTGTTGATCTGGAAGCAGGTAAGCCGACCGTCCGACTGGAGA
ACATCCTGCGGGTTCTTCAGGTGCTGGGTGCAACGCTAAGCGTGTCGAGTTCAGAAGACAGCCAGACACAGGCTGGCCCG
AAATGA

Structures

Toxin

• AlphaFold DB: F4G7M0