Detailed information of TA system TA025193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2173877..2174634
• Replicon: chromosome
• Accession: NC_015422
• Organism: Alicycliphilus denitrificans K601

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: ALIDE2_RS24390
• Protein ID: WP_013518725.1
• Coordinates: 2173877..2174371 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: F4G8U8
• Locus tag: ALIDE2_RS10390
• Protein ID: WP_013518726.1
• Coordinates: 2174368..2174634 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ALIDE2_RS10365	2169282..2170469	-	1188	WP_013518721.1	amidohydrolase	-
ALIDE2_RS10370	2170507..2171826	-	1320	WP_180987920.1	MFS transporter	-
ALIDE2_RS10375	2171974..2172876	+	903	WP_013518723.1	LysR family transcriptional regulator	-
ALIDE2_RS24950	2173006..2173257	-	252	Protein_2061	IS1595 family transposase	-
ALIDE2_RS24390	2173877..2174371	-	495	WP_013518725.1	GNAT family N-acetyltransferase	Toxin
ALIDE2_RS10390	2174368..2174634	-	267	WP_013518726.1	DUF1778 domain-containing protein	Antitoxin
ALIDE2_RS10395	2174865..2176121	-	1257	WP_013518727.1	MFS transporter	-
ALIDE2_RS10400	2176305..2177096	+	792	WP_013518728.1	SDR family oxidoreductase	-
ALIDE2_RS10405	2177160..2177585	+	426	WP_013518729.1	PaaI family thioesterase	-
ALIDE2_RS10410	2177679..2178695	+	1017	WP_013722042.1	NADP-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-80)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17883.59 Da
• Isoelectric Point: 8.4881

>T25193 WP_013518725.1 NC_015422:c2174371-2173877 [Alicycliphilus denitrificans K601]
MSLDLVAPQPLTSDHHCIDFSCGEPVLDEWLKRRAMANQISGASRTFVAADKEHRVYGYYAMAAGAVSHQLATGSVRRNM
PDPVPVMVLARLAVDTQAQGIKLGGSLLQDAVNRAVNVAQNTGVRALLVHALHERAKLFYEYYGFQASPADPLVLMLRLS
QAKP

Nucleotide

• Length: 495 bp

>T25193 NC_015422:c2174371-2173877 [Alicycliphilus denitrificans K601]
ATGAGCCTTGATCTTGTCGCGCCGCAGCCGCTGACGTCAGATCATCACTGCATCGATTTTTCCTGCGGCGAACCGGTGCT
GGATGAATGGCTCAAGCGTCGGGCGATGGCCAATCAGATCAGCGGAGCCAGTCGCACTTTTGTTGCGGCGGATAAAGAGC
ACCGTGTTTATGGCTACTACGCAATGGCGGCTGGTGCTGTGTCCCATCAGCTCGCCACAGGCAGTGTGCGCAGAAACATG
CCTGATCCGGTGCCCGTCATGGTATTGGCCAGGCTTGCCGTCGACACCCAGGCCCAGGGCATCAAGCTGGGTGGCTCCCT
ACTGCAGGATGCCGTCAACCGGGCGGTGAACGTGGCGCAGAACACGGGCGTTCGTGCCTTGCTGGTTCATGCCCTGCACG
AGCGTGCGAAGTTGTTTTACGAGTATTACGGCTTCCAAGCATCTCCGGCTGATCCGCTGGTACTGATGCTGCGCCTGAGC
CAGGCCAAGCCGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9927.40 Da
• Isoelectric Point: 5.6458

>AT25193 WP_013518726.1 NC_015422:c2174634-2174368 [Alicycliphilus denitrificans K601]
MRAAAINLRALPEQRDLIDHAANLLGKNRSDFMLEAACERAQSVVLDQVHFTLDADKFQQFVDLLDAPPQPNTGLERLFA
LKPVWNKV

Nucleotide

• Length: 267 bp

>AT25193 NC_015422:c2174634-2174368 [Alicycliphilus denitrificans K601]
ATGCGTGCTGCCGCCATCAATCTGCGTGCTCTGCCCGAGCAGCGTGACCTCATCGATCACGCCGCCAACCTGCTGGGAAA
GAACCGTTCGGACTTTATGCTGGAGGCCGCCTGCGAGCGTGCCCAGTCTGTTGTGCTTGATCAGGTTCACTTCACGCTGG
ATGCTGACAAATTCCAGCAGTTTGTCGATCTACTCGATGCGCCACCCCAGCCCAACACCGGGCTGGAACGCCTGTTTGCA
CTGAAGCCTGTCTGGAACAAGGTATGA

Structures

Antitoxin

• AlphaFold DB: F4G8U8