Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4931940..4932437 Replicon chromosome
Accession NZ_CP101310
Organism Escherichia coli strain 99-3311

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NMK62_RS24475 Protein ID WP_001173343.1
Coordinates 4931940..4932113 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NMK62_RS24480 Protein ID WP_000398619.1
Coordinates 4932141..4932437 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK62_RS24465 (4928610) 4928610..4930067 + 1458 WP_000856822.1 dipeptide/tripeptide permease DtpC -
NMK62_RS24470 (4930304) 4930304..4931821 + 1518 WP_001295074.1 lysine--tRNA ligase -
NMK62_RS24475 (4931940) 4931940..4932113 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NMK62_RS24480 (4932141) 4932141..4932437 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NMK62_RS24485 (4932664) 4932664..4932936 - 273 WP_000405653.1 GNAT family N-acetyltransferase -
NMK62_RS24490 (4932948) 4932948..4933178 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
NMK62_RS24495 (4933359) 4933359..4934990 + 1632 WP_022581427.1 sensor histidine kinase -
NMK62_RS24500 (4934987) 4934987..4935706 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T251898 WP_001173343.1 NZ_CP101310:c4932113-4931940 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT251898 WP_000398619.1 NZ_CP101310:c4932437-4932141 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References