Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 346653..347453 Replicon chromosome
Accession NZ_CP101310
Organism Escherichia coli strain 99-3311

Toxin (Protein)


Gene name ataT Uniprot ID U9Y257
Locus tag NMK62_RS01690 Protein ID WP_000342447.1
Coordinates 346926..347453 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID U9Y285
Locus tag NMK62_RS01685 Protein ID WP_001277109.1
Coordinates 346653..346919 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK62_RS01665 (342311) 342311..342979 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
NMK62_RS01670 (342972) 342972..344030 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
NMK62_RS01675 (344275) 344275..345129 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
NMK62_RS01680 (345400) 345400..346503 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NMK62_RS01685 (346653) 346653..346919 + 267 WP_001277109.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NMK62_RS01690 (346926) 346926..347453 + 528 WP_000342447.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NMK62_RS01695 (347450) 347450..347833 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
NMK62_RS01700 (348258) 348258..349367 + 1110 WP_001400431.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NMK62_RS01705 (349415) 349415..350341 + 927 WP_000003013.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NMK62_RS01710 (350338) 350338..351615 + 1278 WP_000803782.1 branched chain amino acid ABC transporter permease LivM -
NMK62_RS01715 (351612) 351612..352379 + 768 WP_000082096.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.63 Da        Isoelectric Point: 7.0284

>T251880 WP_000342447.1 NZ_CP101310:346926-347453 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDHSIQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T251880 NZ_CP101310:346926-347453 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCATTCAATACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCAGTAGGTATTCACGGTCTTTTTGTAG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCGTTGGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9938.37 Da        Isoelectric Point: 10.0863

>AT251880 WP_001277109.1 NZ_CP101310:346653-346919 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AVKRLQGM

Download         Length: 267 bp

>AT251880 NZ_CP101310:346653-346919 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGTAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LUM2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LUN0

References