251873

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 4057632..4058469 Replicon chromosome
Accession NZ_CP101305
Organism Escherichia coli strain 2002-3211

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag NMK57_RS20575 Protein ID WP_000227784.1
Coordinates 4057927..4058469 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag NMK57_RS20570 Protein ID WP_001297137.1
Coordinates 4057632..4057943 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK57_RS20545 (4052652) 4052652..4053599 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
NMK57_RS20550 (4053621) 4053621..4055612 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
NMK57_RS20555 (4055602) 4055602..4056216 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
NMK57_RS20560 (4056216) 4056216..4056545 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NMK57_RS20565 (4056557) 4056557..4057447 + 891 WP_000971336.1 heme o synthase -
NMK57_RS20570 (4057632) 4057632..4057943 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
NMK57_RS20575 (4057927) 4057927..4058469 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
NMK57_RS20580 (4058525) 4058525..4059460 - 936 WP_001297127.1 tetratricopeptide repeat protein -
NMK57_RS20585 (4059868) 4059868..4061232 + 1365 WP_001000945.1 MFS transporter -
NMK57_RS20590 (4061360) 4061360..4061851 - 492 WP_001138904.1 nucleotide binding protein YajQ -
NMK57_RS20595 (4062019) 4062019..4062930 + 912 WP_000705865.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T251873 WP_000227784.1 NZ_CP101305:4057927-4058469 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT251873 WP_001297137.1 NZ_CP101305:4057632-4057943 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References