Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 986247..986907 Replicon chromosome
Accession NZ_CP101305
Organism Escherichia coli strain 2002-3211

Toxin (Protein)


Gene name cptA Uniprot ID A0A3W5XVE6
Locus tag NMK57_RS04950 Protein ID WP_000244778.1
Coordinates 986494..986907 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NMK57_RS04945 Protein ID WP_000354046.1
Coordinates 986247..986513 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK57_RS04920 (981535) 981535..982278 + 744 WP_000951948.1 SDR family oxidoreductase -
NMK57_RS04925 (982335) 982335..983768 - 1434 WP_001343615.1 6-phospho-beta-glucosidase BglA -
NMK57_RS04930 (983813) 983813..984124 + 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
NMK57_RS04935 (984288) 984288..984947 + 660 WP_000250274.1 hemolysin III family protein -
NMK57_RS04940 (985024) 985024..986004 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NMK57_RS04945 (986247) 986247..986513 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NMK57_RS04950 (986494) 986494..986907 + 414 WP_000244778.1 protein YgfX Toxin
NMK57_RS04955 (986941) 986941..987462 - 522 WP_001055874.1 flavodoxin FldB -
NMK57_RS04960 (987574) 987574..988470 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NMK57_RS04965 (988494) 988494..989204 + 711 WP_000715231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NMK57_RS04970 (989210) 989210..990943 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16275.23 Da        Isoelectric Point: 11.7064

>T251860 WP_000244778.1 NZ_CP101305:986494-986907 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQRSR

Download         Length: 414 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT251860 WP_000354046.1 NZ_CP101305:986247-986513 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W5XVE6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References