Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4466924..4467578 Replicon chromosome
Accession NZ_CP101302
Organism Escherichia coli strain 2000-3039

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag NMK59_RS22465 Protein ID WP_000244772.1
Coordinates 4466924..4467331 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NMK59_RS22470 Protein ID WP_000354046.1
Coordinates 4467312..4467578 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK59_RS22445 (4462881) 4462881..4464614 - 1734 WP_000813223.1 single-stranded-DNA-specific exonuclease RecJ -
NMK59_RS22450 (4464620) 4464620..4465330 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NMK59_RS22455 (4465355) 4465355..4466251 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NMK59_RS22460 (4466363) 4466363..4466884 + 522 WP_001055874.1 flavodoxin FldB -
NMK59_RS22465 (4466924) 4466924..4467331 - 408 WP_000244772.1 protein YgfX Toxin
NMK59_RS22470 (4467312) 4467312..4467578 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NMK59_RS22475 (4467821) 4467821..4468801 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NMK59_RS22480 (4469054) 4469054..4470034 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
NMK59_RS22485 (4470157) 4470157..4470816 - 660 WP_000250274.1 hemolysin III family protein -
NMK59_RS22490 (4470980) 4470980..4471291 - 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4469054..4470034 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T251848 WP_000244772.1 NZ_CP101302:c4467331-4466924 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT251848 WP_000354046.1 NZ_CP101302:c4467578-4467312 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References