Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4297928..4298511 Replicon chromosome
Accession NZ_CP101302
Organism Escherichia coli strain 2000-3039

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag NMK59_RS21730 Protein ID WP_023982871.1
Coordinates 4297928..4298263 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag NMK59_RS21735 Protein ID WP_000581937.1
Coordinates 4298263..4298511 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK59_RS21715 (4293815) 4293815..4295113 - 1299 WP_000036723.1 phosphopyruvate hydratase -
NMK59_RS21720 (4295201) 4295201..4296838 - 1638 WP_000210883.1 CTP synthase (glutamine hydrolyzing) -
NMK59_RS21725 (4297066) 4297066..4297857 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
NMK59_RS21730 (4297928) 4297928..4298263 - 336 WP_023982871.1 endoribonuclease MazF Toxin
NMK59_RS21735 (4298263) 4298263..4298511 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
NMK59_RS21740 (4298589) 4298589..4300823 - 2235 WP_000226815.1 GTP pyrophosphokinase -
NMK59_RS21745 (4300871) 4300871..4302172 - 1302 WP_000046800.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12172.10 Da        Isoelectric Point: 8.4777

>T251847 WP_023982871.1 NZ_CP101302:c4298263-4297928 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCIPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT251847 WP_000581937.1 NZ_CP101302:c4298511-4298263 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References