Detailed information of TA system TA025184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 802790..803355
• Replicon: chromosome
• Accession: NC_015385
• Organism: Treponema succinifaciens DSM 2489

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: F2NX70
• Locus tag: TRESU_RS03780
• Protein ID: WP_013700981.1
• Coordinates: 803017..803355 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: F2NX69
• Locus tag: TRESU_RS03775
• Protein ID: WP_013700980.1
• Coordinates: 802790..803023 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TRESU_RS03735	797790..798278	+	489	WP_013700973.1	hypothetical protein	-
TRESU_RS03740	798407..798956	+	550	Protein_741	hypothetical protein	-
TRESU_RS03745	799325..800539	-	1215	WP_013700952.1	IS110 family transposase	-
TRESU_RS03750	800766..801101	+	336	WP_013700975.1	TM2 domain-containing protein	-
TRESU_RS03755	801166..801426	+	261	WP_013700976.1	DUF2752 domain-containing protein	-
TRESU_RS03760	801503..801730	+	228	WP_013700977.1	DUF2281 domain-containing protein	-
TRESU_RS03765	801727..802104	+	378	WP_013700978.1	type II toxin-antitoxin system VapC family toxin	-
TRESU_RS03770	802348..802689	+	342	WP_013700979.1	hypothetical protein	-
TRESU_RS03775	802790..803023	+	234	WP_013700980.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TRESU_RS03780	803017..803355	+	339	WP_013700981.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TRESU_RS03785	803727..804942	-	1216	Protein_750	IS110 family transposase	-
TRESU_RS03795	805151..805771	+	621	WP_013700982.1	DUF4157 domain-containing protein	-
TRESU_RS03800	805776..805973	+	198	WP_013700983.1	hypothetical protein	-
TRESU_RS14865	806207..806881	+	675	Protein_753	hypothetical protein	-
TRESU_RS14870	806915..807397	+	483	WP_041612201.1	DNA/RNA non-specific endonuclease	-
TRESU_RS03810	807417..807866	+	450	WP_013700985.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	745928..846372	100444

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-110)

Antitoxin

• SpoVT-AbrB domain (6-47)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12844.75 Da
• Isoelectric Point: 6.0685

>T25184 WP_013700981.1 NC_015385:803017-803355 [Treponema succinifaciens DSM 2489]
MVNLNYVPEKGDLVWLDFNPQAGHEQKGRCPAICISQKKYNQKIGLALFCPITSHIKGYPFEIVLDNHSINGCILSDQVK
NLDYRQRHCDFIEKATEEEINSVIDNIKLLIE

Nucleotide

• Length: 339 bp

>T25184 NC_015385:803017-803355 [Treponema succinifaciens DSM 2489]
ATGGTAAATTTAAATTATGTTCCTGAAAAAGGTGATTTAGTCTGGCTCGATTTTAATCCGCAAGCAGGACATGAACAAAA
AGGTCGCTGCCCTGCAATTTGTATATCTCAGAAAAAATACAATCAGAAAATTGGACTTGCTCTATTCTGCCCTATAACAA
GCCATATCAAGGGATATCCTTTTGAAATTGTATTGGACAATCATTCAATAAATGGTTGCATCTTAAGCGATCAGGTAAAA
AATCTTGATTATAGACAAAGACATTGTGATTTTATAGAAAAAGCGACAGAAGAGGAAATTAATTCTGTTATAGATAATAT
AAAACTGTTAATAGAATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8542.74 Da
• Isoelectric Point: 5.0351

>AT25184 WP_013700980.1 NC_015385:802790-803023 [Treponema succinifaciens DSM 2489]
MEAVVQKWGNSLGFRIPSLWAKDNNVKNGSKIEVIAEKGKIVILPQKKTLDDMLDMVTSENIHSEISTGSSVGNEEW

Nucleotide

• Length: 234 bp

>AT25184 NC_015385:802790-803023 [Treponema succinifaciens DSM 2489]
ATGGAAGCTGTAGTTCAGAAATGGGGTAACAGTCTCGGTTTTAGAATTCCGTCACTTTGGGCAAAAGACAACAATGTAAA
GAACGGTAGTAAAATTGAAGTAATCGCTGAAAAAGGAAAAATAGTAATTCTTCCTCAGAAAAAAACTCTTGATGATATGC
TGGATATGGTTACTTCAGAAAACATTCATTCAGAAATATCAACCGGCAGTTCGGTAGGTAATGAAGAATGGTAA

Structures

Toxin

• AlphaFold DB: F2NX70

Antitoxin

• AlphaFold DB: F2NX69