Detailed information of TA system TA025183

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 243683..244260
• Replicon: plasmid bgla_1p
• Accession: NC_015382
• Organism: Burkholderia gladioli BSR3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F2LRT1
• Locus tag: BGLA_RS35495
• Protein ID: WP_013699949.1
• Coordinates: 243683..244048 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F2LRT2
• Locus tag: BGLA_RS35500
• Protein ID: WP_013699950.1
• Coordinates: 244042..244260 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGLA_RS42645	239883..240035	-	153	WP_160167562.1	hypothetical protein	-
BGLA_RS35480	240590..241725	+	1136	WP_085963582.1	IS3 family transposase	-
BGLA_RS42215	241709..241909	-	201	WP_148270661.1	hypothetical protein	-
BGLA_RS35490	242479..243138	-	660	WP_044275745.1	hypothetical protein	-
BGLA_RS40740	243227..243439	-	213	WP_013699948.1	hypothetical protein	-
BGLA_RS42220	243457..243639	-	183	WP_148270663.1	hypothetical protein	-
BGLA_RS35495	243683..244048	-	366	WP_013699949.1	type II toxin-antitoxin system VapC family toxin	Toxin
BGLA_RS35500	244042..244260	-	219	WP_013699950.1	ribbon-helix-helix protein, CopG family	Antitoxin
BGLA_RS35505	244726..245424	+	699	WP_044275746.1	hypothetical protein	-
BGLA_RS35510	245468..246508	-	1041	WP_044275747.1	hypothetical protein	-
BGLA_RS35515	246757..246963	-	207	WP_044275748.1	hypothetical protein	-
BGLA_RS35520	246996..247424	-	429	WP_013699952.1	type II toxin-antitoxin system VapC family toxin	-
BGLA_RS35525	247424..247681	-	258	WP_013699953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
BGLA_RS35530	247745..248404	-	660	WP_013699954.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fliC	1..276215	276215

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-41)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13467.63 Da
• Isoelectric Point: 8.4446

>T25183 WP_013699949.1 NC_015382:c244048-243683 [Burkholderia gladioli BSR3]
MVKALFDTNILIDYLGGIAAAKKELGRYEYRAISTITWMEVLVGASSGEEDAIRGWLDSFDVIPVGSDIANRAVVIRKAR
RIRLPDAIIWATAQVHSLLLVSRNTKDFPPDEPGVRIPYKL

Nucleotide

• Length: 366 bp

>T25183 NC_015382:c244048-243683 [Burkholderia gladioli BSR3]
ATGGTAAAGGCGCTGTTTGACACGAATATCCTCATCGACTATCTCGGCGGCATCGCCGCCGCGAAGAAGGAGCTGGGGCG
CTATGAGTATCGTGCGATCAGCACCATTACGTGGATGGAAGTGCTTGTTGGTGCGTCATCAGGCGAAGAAGATGCGATCC
GGGGGTGGCTCGACTCATTCGACGTTATTCCGGTGGGCAGCGACATAGCGAATCGCGCGGTCGTGATTCGCAAGGCCAGA
CGGATTCGTTTGCCGGATGCCATCATTTGGGCCACGGCGCAAGTGCATTCCCTGTTGCTCGTGTCACGCAATACGAAGGA
TTTTCCACCAGATGAACCGGGCGTGCGAATTCCCTATAAGTTGTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8169.35 Da
• Isoelectric Point: 5.0490

>AT25183 WP_013699950.1 NC_015382:c244260-244042 [Burkholderia gladioli BSR3]
MSRILVDLPDSQVDDLAVIVEAEQRPRAAVIRDAIEAYIAQRKRALGADVFGLWKSKKVDGLAYQQELRSEW

Nucleotide

• Length: 219 bp

>AT25183 NC_015382:c244260-244042 [Burkholderia gladioli BSR3]
ATGAGCCGAATCCTAGTTGACCTGCCCGATTCGCAGGTCGATGACCTCGCAGTCATCGTTGAGGCGGAGCAACGTCCCCG
AGCGGCCGTTATCCGCGACGCCATTGAGGCATACATCGCTCAACGCAAACGCGCACTTGGCGCCGATGTGTTCGGACTGT
GGAAAAGCAAGAAGGTTGACGGCCTAGCGTATCAACAGGAGCTTCGTTCAGAATGGTAA

Structures

Toxin

• AlphaFold DB: F2LRT1

Antitoxin

• AlphaFold DB: F2LRT2