251829

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1312902..1313739 Replicon chromosome
Accession NZ_CP101302
Organism Escherichia coli strain 2000-3039

Toxin (Protein)


Gene name itaT Uniprot ID A0A0F5GET7
Locus tag NMK59_RS06175 Protein ID WP_000227783.1
Coordinates 1312902..1313444 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag NMK59_RS06180 Protein ID WP_001297137.1
Coordinates 1313428..1313739 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK59_RS06155 (1308441) 1308441..1309352 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
NMK59_RS06160 (1309520) 1309520..1310011 + 492 WP_001138904.1 nucleotide binding protein YajQ -
NMK59_RS06165 (1310139) 1310139..1311503 - 1365 WP_001000978.1 MFS transporter -
NMK59_RS06170 (1311911) 1311911..1312846 + 936 WP_001434881.1 tetratricopeptide repeat protein -
NMK59_RS06175 (1312902) 1312902..1313444 - 543 WP_000227783.1 GNAT family N-acetyltransferase Toxin
NMK59_RS06180 (1313428) 1313428..1313739 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
NMK59_RS06185 (1313924) 1313924..1314814 - 891 WP_000971336.1 heme o synthase -
NMK59_RS06190 (1314826) 1314826..1315155 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NMK59_RS06195 (1315155) 1315155..1315769 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
NMK59_RS06200 (1315759) 1315759..1317750 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
NMK59_RS06205 (1317772) 1317772..1318719 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19776.96 Da        Isoelectric Point: 8.0330

>T251829 WP_000227783.1 NZ_CP101302:c1313444-1312902 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT251829 WP_001297137.1 NZ_CP101302:c1313739-1313428 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F5GET7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References