Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 477042..477539 Replicon chromosome
Accession NZ_CP101302
Organism Escherichia coli strain 2000-3039

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NMK59_RS02180 Protein ID WP_001173343.1
Coordinates 477366..477539 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NMK59_RS02175 Protein ID WP_000398619.1
Coordinates 477042..477338 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK59_RS02155 (473773) 473773..474492 - 720 WP_000611288.1 two-component system response regulator DcuR -
NMK59_RS02160 (474489) 474489..476120 - 1632 WP_001216487.1 sensor histidine kinase -
NMK59_RS02165 (476301) 476301..476531 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
NMK59_RS02170 (476543) 476543..476815 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
NMK59_RS02175 (477042) 477042..477338 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NMK59_RS02180 (477366) 477366..477539 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NMK59_RS02185 (477658) 477658..479175 - 1518 WP_001295074.1 lysine--tRNA ligase -
NMK59_RS02190 (479412) 479412..480869 - 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T251824 WP_001173343.1 NZ_CP101302:477366-477539 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT251824 WP_000398619.1 NZ_CP101302:477042-477338 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References