Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 4715700..4716283 Replicon chromosome
Accession NZ_CP101292
Organism Escherichia coli strain 2003-3014

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag NMK58_RS24490 Protein ID WP_000254745.1
Coordinates 4715700..4716035 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag NMK58_RS24495 Protein ID WP_000581937.1
Coordinates 4716035..4716283 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK58_RS24475 (4711587) 4711587..4712885 - 1299 WP_000036723.1 phosphopyruvate hydratase -
NMK58_RS24480 (4712973) 4712973..4714610 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
NMK58_RS24485 (4714838) 4714838..4715629 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
NMK58_RS24490 (4715700) 4715700..4716035 - 336 WP_000254745.1 endoribonuclease MazF Toxin
NMK58_RS24495 (4716035) 4716035..4716283 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
NMK58_RS24500 (4716361) 4716361..4718595 - 2235 WP_000226815.1 GTP pyrophosphokinase -
NMK58_RS24505 (4718643) 4718643..4719944 - 1302 WP_000046800.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T251819 WP_000254745.1 NZ_CP101292:c4716035-4715700 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT251819 WP_000581937.1 NZ_CP101292:c4716283-4716035 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References