Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1271191..1272028 Replicon chromosome
Accession NZ_CP101292
Organism Escherichia coli strain 2003-3014

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag NMK58_RS06070 Protein ID WP_000227784.1
Coordinates 1271191..1271733 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag NMK58_RS06075 Protein ID WP_001297137.1
Coordinates 1271717..1272028 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMK58_RS06050 (1266730) 1266730..1267641 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
NMK58_RS06055 (1267809) 1267809..1268300 + 492 WP_001138904.1 nucleotide binding protein YajQ -
NMK58_RS06060 (1268428) 1268428..1269792 - 1365 WP_001000977.1 MFS transporter -
NMK58_RS06065 (1270200) 1270200..1271135 + 936 WP_001375746.1 tetratricopeptide repeat protein -
NMK58_RS06070 (1271191) 1271191..1271733 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
NMK58_RS06075 (1271717) 1271717..1272028 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
NMK58_RS06080 (1272213) 1272213..1273103 - 891 WP_000971336.1 heme o synthase -
NMK58_RS06085 (1273115) 1273115..1273444 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NMK58_RS06090 (1273444) 1273444..1274058 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
NMK58_RS06095 (1274048) 1274048..1276039 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
NMK58_RS06100 (1276061) 1276061..1277008 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T251800 WP_000227784.1 NZ_CP101292:c1271733-1271191 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT251800 WP_001297137.1 NZ_CP101292:c1272028-1271717 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References