251792

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1327827..1328743 Replicon chromosome
Accession NZ_CP101290
Organism Bacillus subtilis strain LjM2

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag NMT99_RS06960 Protein ID WP_003244695.1
Coordinates 1327997..1328743 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag NMT99_RS06955 Protein ID WP_003232646.1
Coordinates 1327827..1327997 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMT99_RS06920 1324693..1325019 + 327 WP_069703538.1 XkdW family protein -
NMT99_RS06925 1325016..1325180 + 165 WP_014479563.1 XkdX family protein -
NMT99_RS06930 1325224..1326063 + 840 WP_029727045.1 phage-like element PBSX protein XepA -
NMT99_RS06935 1326116..1326385 + 270 WP_015252265.1 hemolysin XhlA family protein -
NMT99_RS06940 1326398..1326661 + 264 WP_014479566.1 phage holin -
NMT99_RS06945 1326674..1327567 + 894 WP_029727044.1 N-acetylmuramoyl-L-alanine amidase -
NMT99_RS06950 1327604..1327741 - 138 WP_119899277.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
NMT99_RS06955 1327827..1327997 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
NMT99_RS06960 1327997..1328743 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
NMT99_RS06965 1328853..1329854 - 1002 WP_003232642.1 inorganic phosphate transporter -
NMT99_RS06970 1329867..1330484 - 618 WP_003218470.1 DUF47 domain-containing protein -
NMT99_RS06975 1330760..1332076 - 1317 WP_015252262.1 serine/threonine exchanger -
NMT99_RS06980 1332465..1333415 + 951 WP_014476561.1 ring-cleaving dioxygenase -
NMT99_RS06985 1333524..1333619 + 96 Protein_1312 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T251792 WP_003244695.1 NZ_CP101290:c1328743-1327997 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT251792 WP_003232646.1 NZ_CP101290:c1327997-1327827 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References