Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 516626..517262 Replicon chromosome
Accession NZ_CP101290
Organism Bacillus subtilis strain LjM2

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag NMT99_RS02630 Protein ID WP_003156187.1
Coordinates 516912..517262 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag NMT99_RS02625 Protein ID WP_003225183.1
Coordinates 516626..516907 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NMT99_RS02605 512985..513584 - 600 WP_003246687.1 rhomboid family intramembrane serine protease -
NMT99_RS02610 513679..514044 + 366 WP_015252768.1 holo-ACP synthase -
NMT99_RS02615 514210..515226 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
NMT99_RS02620 515341..516510 + 1170 WP_003234284.1 alanine racemase -
NMT99_RS02625 516626..516907 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
NMT99_RS02630 516912..517262 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NMT99_RS02635 517377..518201 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
NMT99_RS02640 518206..518571 + 366 WP_015715277.1 RsbT antagonist protein RsbS -
NMT99_RS02645 518575..518976 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
NMT99_RS02650 518988..519995 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
NMT99_RS02655 520057..520386 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
NMT99_RS02660 520383..520865 + 483 WP_003234299.1 anti-sigma B factor RsbW -
NMT99_RS02665 520831..521619 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
NMT99_RS02670 521619..522218 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T251791 WP_003156187.1 NZ_CP101290:516912-517262 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT251791 WP_003225183.1 NZ_CP101290:516626-516907 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References