Detailed information of TA system TA025179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4901457..4902045
• Replicon: chromosome
• Accession: NC_015379
• Organism: Pseudomonas brassicacearum subsp. brassicacearum NFM421

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: PSEBR_RS21390
• Protein ID: WP_162470867.1
• Coordinates: 4901457..4901699 (-)
• Length: 81 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F2KK14
• Locus tag: PSEBR_RS21395
• Protein ID: WP_013693974.1
• Coordinates: 4901758..4902045 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PSEBR_RS21360	4897393..4897881	-	489	WP_013693967.1	GNAT family N-acetyltransferase	-
PSEBR_RS21365	4898212..4899015	-	804	WP_013693968.1	diaminopimelate epimerase	-
PSEBR_RS21370	4899006..4899371	-	366	WP_013693969.1	VOC family protein	-
PSEBR_RS21375	4899430..4899993	-	564	WP_013693970.1	GNAT family N-acetyltransferase	-
PSEBR_RS21380	4900043..4900627	-	585	WP_013693971.1	hypothetical protein	-
PSEBR_RS21385	4900811..4901452	-	642	WP_041931234.1	transcriptional regulator	-
PSEBR_RS21390	4901457..4901699	-	243	WP_162470867.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PSEBR_RS21395	4901758..4902045	-	288	WP_013693974.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PSEBR_RS21400	4902197..4903153	-	957	WP_013693975.1	2-hydroxyacid dehydrogenase	-
PSEBR_RS21405	4903186..4903944	-	759	WP_003204148.1	SDR family oxidoreductase	-
PSEBR_RS21410	4903966..4904664	-	699	WP_013693976.1	fumarylacetoacetate hydrolase family protein	-
PSEBR_RS21415	4904766..4906115	-	1350	WP_003204153.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-62)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-72)

Sequences

Toxin

Protein

• Length: 81 a.a.
• Molecular weight: 9126.67 Da
• Isoelectric Point: 10.7176

>T25179 WP_162470867.1 NC_015379:c4901699-4901457 [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
IRLQLLKKLQTRQGNPRVPGDALHGMKDCYKIKLRGAGYRLVYRVEDERIVILVVAIGKRERGNVYDSAKDRASMQDTKG

Nucleotide

• Length: 243 bp

>T25179 NC_015379:c4901699-4901457 [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
ATCCGTCTGCAGCTTCTGAAGAAACTCCAGACCCGGCAGGGCAACCCTCGAGTGCCCGGCGACGCGCTTCATGGAATGAA
GGATTGCTACAAGATCAAATTGCGCGGGGCTGGGTATCGACTGGTGTACCGCGTTGAGGATGAGCGGATTGTCATCCTGG
TGGTAGCGATTGGCAAACGGGAGCGTGGCAACGTTTACGATTCGGCCAAGGACCGGGCTAGTATGCAAGACACAAAGGGG
TAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10233.72 Da
• Isoelectric Point: 4.3219

>AT25179 WP_013693974.1 NC_015379:c4902045-4901758 [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
MQIVLADVAVSVSELKKNPSSVLNGANGAPVAVLNHNRVMGYMVPASVYEAMVDRLEELELAQVVKARIEANETPVRVSF
DELIGEAEADIINGR

Nucleotide

• Length: 288 bp

>AT25179 NC_015379:c4902045-4901758 [Pseudomonas brassicacearum subsp. brassicacearum NFM421]
ATGCAAATCGTTTTGGCTGATGTGGCCGTCAGCGTATCCGAACTCAAAAAAAACCCATCATCCGTCCTTAACGGCGCCAA
CGGCGCGCCGGTGGCGGTGTTGAACCATAACCGGGTCATGGGCTACATGGTCCCGGCTAGTGTCTACGAGGCGATGGTTG
ATCGCTTGGAAGAACTGGAATTGGCGCAAGTGGTCAAGGCACGCATCGAGGCCAACGAAACACCTGTTCGGGTGTCTTTT
GACGAGCTTATTGGCGAGGCTGAGGCTGATATCATCAATGGGCGTTGA

Structures

Antitoxin

• AlphaFold DB: F2KK14