Detailed information of TA system TA025178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 8896..9572
• Replicon: plasmid bgla_3p
• Accession: NC_015378
• Organism: Burkholderia gladioli BSR3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: BGLA_RS36305
• Protein ID: WP_044275898.1
• Coordinates: 8896..9327 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F2LSB7
• Locus tag: BGLA_RS36310
• Protein ID: WP_013691848.1
• Coordinates: 9351..9572 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BGLA_RS36280	4007..5239	-	1233	WP_013691841.1	tyrosine-type recombinase/integrase	-
BGLA_RS36285	5339..5983	-	645	WP_013691842.1	hypothetical protein	-
BGLA_RS40805	6285..6536	+	252	WP_013691843.1	hypothetical protein	-
BGLA_RS36290	6546..7376	+	831	WP_013691844.1	hypothetical protein	-
BGLA_RS36295	7502..7696	-	195	WP_013691845.1	hypothetical protein	-
BGLA_RS36300	7788..8873	-	1086	WP_013691846.1	hypothetical protein	-
BGLA_RS36305	8896..9327	-	432	WP_044275898.1	PIN domain-containing protein	Toxin
BGLA_RS36310	9351..9572	-	222	WP_013691848.1	hypothetical protein	Antitoxin
BGLA_RS36315	9764..10234	+	471	WP_044275836.1	hypothetical protein	-
BGLA_RS36320	10389..10595	-	207	WP_148270701.1	hypothetical protein	-
BGLA_RS42240	10623..10973	-	351	WP_148270703.1	hypothetical protein	-
BGLA_RS36325	10988..11593	-	606	WP_013691849.1	hypothetical protein	-
BGLA_RS36330	11608..11901	-	294	WP_044275838.1	hypothetical protein	-
BGLA_RS36335	12181..12906	-	726	WP_013691850.1	parR	-
BGLA_RS39035	12979..14109	-	1131	WP_013691851.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..128650	128650

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-127)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15614.80 Da
• Isoelectric Point: 6.0490

>T25178 WP_044275898.1 NC_015378:c9327-8896 [Burkholderia gladioli BSR3]
MTYLLDVNVLIALLDAGHVQHEAAHEWFGSAAHAAWATCPLTENGVLRIIGNPRYPNTLGTPAAVAPLVTQLRAHPGHTF
WWDDLSLLNPEQVDVSRILATDQVTDTYLLALARVHGGKLATFDRRLVVDAVPDGARHLEIIS

Nucleotide

• Length: 432 bp

>T25178 NC_015378:c9327-8896 [Burkholderia gladioli BSR3]
ATGACTTACCTGCTCGACGTCAACGTCCTGATCGCGCTGCTCGATGCGGGCCACGTGCAGCACGAGGCGGCGCACGAATG
GTTTGGCAGCGCCGCGCACGCGGCGTGGGCGACCTGCCCGCTGACCGAAAACGGCGTGCTGCGCATCATCGGCAACCCGC
GCTACCCGAACACGCTGGGCACGCCCGCCGCGGTCGCGCCGCTCGTCACACAGCTGCGCGCGCACCCGGGGCACACGTTC
TGGTGGGACGACCTGAGCCTGCTCAATCCGGAACAGGTCGACGTGAGCCGCATCCTCGCCACCGACCAGGTGACCGACAC
CTACCTGCTCGCGCTCGCGCGCGTGCACGGGGGCAAGCTGGCCACCTTCGACCGACGCCTCGTGGTCGATGCGGTGCCCG
ACGGCGCGCGCCATCTCGAAATCATCAGCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 7851.97 Da
• Isoelectric Point: 7.0108

>AT25178 WP_013691848.1 NC_015378:c9572-9351 [Burkholderia gladioli BSR3]
MRTTLEIDDDVLALARARADREHVSIGRIISQLARAALQPAAAAPTTRNGLPVLPSARTVTPELVNQLRDEAP

Nucleotide

• Length: 222 bp

>AT25178 NC_015378:c9572-9351 [Burkholderia gladioli BSR3]
ATGCGCACCACCCTCGAAATCGACGACGACGTACTGGCGCTCGCTCGCGCGCGCGCCGACCGCGAGCACGTCTCGATCGG
CCGCATCATCTCGCAGCTCGCGCGCGCCGCGCTGCAGCCGGCGGCCGCGGCACCCACCACCCGCAACGGCCTGCCCGTGC
TGCCGAGCGCGCGCACGGTCACGCCCGAGCTCGTCAACCAGCTTCGCGACGAGGCGCCCTGA

Structures

Antitoxin

• AlphaFold DB: F2LSB7