Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4351057..4351857 Replicon chromosome
Accession NZ_CP101274
Organism Escherichia coli strain EC21Z-014

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag NML17_RS21295 Protein ID WP_000342449.1
Coordinates 4351057..4351584 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NML17_RS21300 Protein ID WP_001277108.1
Coordinates 4351591..4351857 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML17_RS21270 (4346132) 4346132..4346899 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
NML17_RS21275 (4346896) 4346896..4348173 - 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
NML17_RS21280 (4348170) 4348170..4349096 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NML17_RS21285 (4349144) 4349144..4350253 - 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NML17_RS21290 (4350677) 4350677..4351060 + 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
NML17_RS21295 (4351057) 4351057..4351584 - 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NML17_RS21300 (4351591) 4351591..4351857 - 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NML17_RS21305 (4352007) 4352007..4353110 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NML17_RS21310 (4353381) 4353381..4354235 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
NML17_RS21315 (4354480) 4354480..4355538 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
NML17_RS21320 (4355531) 4355531..4356199 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T251775 WP_000342449.1 NZ_CP101274:c4351584-4351057 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT251775 WP_001277108.1 NZ_CP101274:c4351857-4351591 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References