Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1050046..1050883 Replicon chromosome
Accession NZ_CP101274
Organism Escherichia coli strain EC21Z-014

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag NML17_RS05010 Protein ID WP_000227784.1
Coordinates 1050046..1050588 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag NML17_RS05015 Protein ID WP_001297137.1
Coordinates 1050572..1050883 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML17_RS04990 (1045585) 1045585..1046496 - 912 WP_000705847.1 2-dehydropantoate 2-reductase -
NML17_RS04995 (1046664) 1046664..1047155 + 492 WP_001138904.1 nucleotide binding protein YajQ -
NML17_RS05000 (1047283) 1047283..1048647 - 1365 WP_001000978.1 MFS transporter -
NML17_RS05005 (1049055) 1049055..1049990 + 936 WP_001297127.1 tetratricopeptide repeat protein -
NML17_RS05010 (1050046) 1050046..1050588 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
NML17_RS05015 (1050572) 1050572..1050883 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
NML17_RS05020 (1051068) 1051068..1051958 - 891 WP_000971336.1 heme o synthase -
NML17_RS05025 (1051970) 1051970..1052299 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NML17_RS05030 (1052299) 1052299..1052913 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
NML17_RS05035 (1052903) 1052903..1054894 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
NML17_RS05040 (1054916) 1054916..1055863 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T251759 WP_000227784.1 NZ_CP101274:c1050588-1050046 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT251759 WP_001297137.1 NZ_CP101274:c1050883-1050572 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References