251757

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 435189..436524 Replicon chromosome
Accession NZ_CP101274
Organism Escherichia coli strain EC21Z-014

Toxin (Protein)


Gene name sanaT Uniprot ID F4VMR9
Locus tag NML17_RS02050 Protein ID WP_000342497.1
Coordinates 435577..436524 (+) Length 316 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID A0A228Z6F8
Locus tag NML17_RS02045 Protein ID WP_000152739.1
Coordinates 435189..435584 (+) Length 132 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML17_RS02030 (431261) 431261..432427 - 1167 WP_001293040.1 restriction endonuclease subunit S -
NML17_RS02035 (432424) 432424..433980 - 1557 WP_000202816.1 type I restriction-modification system subunit M -
NML17_RS02040 (434286) 434286..435119 + 834 WP_001192741.1 DUF4942 domain-containing protein -
NML17_RS02045 (435189) 435189..435584 + 396 WP_000152739.1 DUF6088 family protein Antitoxin
NML17_RS02050 (435577) 435577..436524 + 948 WP_000342497.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
NML17_RS02055 (436562) 436562..436747 - 186 WP_000066585.1 hypothetical protein -
NML17_RS02060 (436846) 436846..436989 - 144 Protein_394 HNH endonuclease -
NML17_RS02065 (437126) 437126..437776 + 651 WP_001037963.1 HNH endonuclease -
NML17_RS02070 (437784) 437784..438764 - 981 WP_000991446.1 sialate O-acetylesterase -
NML17_RS02075 (438829) 438829..439935 - 1107 WP_001298927.1 N-acetylneuraminate epimerase -
NML17_RS02080 (439955) 439955..440671 - 717 WP_001295734.1 N-acetylneuraminic acid outer membrane channel NanC -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fimB / fimE 435189..443803 8614


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 316 a.a.        Molecular weight: 35588.79 Da        Isoelectric Point: 4.9737

>T251757 WP_000342497.1 NZ_CP101274:435577-436524 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Download         Length: 948 bp


Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14605.90 Da        Isoelectric Point: 11.6915

>AT251757 WP_000152739.1 NZ_CP101274:435189-435584 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRDLVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Download         Length: 396 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A1L5BAQ6


Antitoxin

Source ID Structure
AlphaFold DB A0A228Z6F8

References