Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | sanaTA/AbiEii-DUF6088 |
| Location | 435189..436524 | Replicon | chromosome |
| Accession | NZ_CP101274 | ||
| Organism | Escherichia coli strain EC21Z-014 | ||
Toxin (Protein)
| Gene name | sanaT | Uniprot ID | F4VMR9 |
| Locus tag | NML17_RS02050 | Protein ID | WP_000342497.1 |
| Coordinates | 435577..436524 (+) | Length | 316 a.a. |
Antitoxin (Protein)
| Gene name | sanaA | Uniprot ID | A0A228Z6F8 |
| Locus tag | NML17_RS02045 | Protein ID | WP_000152739.1 |
| Coordinates | 435189..435584 (+) | Length | 132 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NML17_RS02030 (431261) | 431261..432427 | - | 1167 | WP_001293040.1 | restriction endonuclease subunit S | - |
| NML17_RS02035 (432424) | 432424..433980 | - | 1557 | WP_000202816.1 | type I restriction-modification system subunit M | - |
| NML17_RS02040 (434286) | 434286..435119 | + | 834 | WP_001192741.1 | DUF4942 domain-containing protein | - |
| NML17_RS02045 (435189) | 435189..435584 | + | 396 | WP_000152739.1 | DUF6088 family protein | Antitoxin |
| NML17_RS02050 (435577) | 435577..436524 | + | 948 | WP_000342497.1 | nucleotidyl transferase AbiEii/AbiGii toxin family protein | Toxin |
| NML17_RS02055 (436562) | 436562..436747 | - | 186 | WP_000066585.1 | hypothetical protein | - |
| NML17_RS02060 (436846) | 436846..436989 | - | 144 | Protein_394 | HNH endonuclease | - |
| NML17_RS02065 (437126) | 437126..437776 | + | 651 | WP_001037963.1 | HNH endonuclease | - |
| NML17_RS02070 (437784) | 437784..438764 | - | 981 | WP_000991446.1 | sialate O-acetylesterase | - |
| NML17_RS02075 (438829) | 438829..439935 | - | 1107 | WP_001298927.1 | N-acetylneuraminate epimerase | - |
| NML17_RS02080 (439955) | 439955..440671 | - | 717 | WP_001295734.1 | N-acetylneuraminic acid outer membrane channel NanC | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | fimB / fimE | 435189..443803 | 8614 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 316 a.a. Molecular weight: 35588.79 Da Isoelectric Point: 4.9737
>T251757 WP_000342497.1 NZ_CP101274:435577-436524 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA
Download Length: 948 bp
Antitoxin
Download Length: 132 a.a. Molecular weight: 14605.90 Da Isoelectric Point: 11.6915
>AT251757 WP_000152739.1 NZ_CP101274:435189-435584 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRDLVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRDLVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG
Download Length: 396 bp
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1L5BAQ6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A228Z6F8 |