Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 280827..281324 Replicon chromosome
Accession NZ_CP101274
Organism Escherichia coli strain EC21Z-014

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NML17_RS01285 Protein ID WP_001173343.1
Coordinates 281151..281324 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NML17_RS01280 Protein ID WP_000398619.1
Coordinates 280827..281123 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML17_RS01260 (277558) 277558..278277 - 720 WP_000611288.1 two-component system response regulator DcuR -
NML17_RS01265 (278274) 278274..279905 - 1632 WP_001216461.1 sensor histidine kinase -
NML17_RS01270 (280086) 280086..280316 + 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
NML17_RS01275 (280328) 280328..280600 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
NML17_RS01280 (280827) 280827..281123 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NML17_RS01285 (281151) 281151..281324 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NML17_RS01290 (281443) 281443..282960 - 1518 WP_001295074.1 lysine--tRNA ligase -
NML17_RS01295 (283197) 283197..284654 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T251755 WP_001173343.1 NZ_CP101274:281151-281324 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT251755 WP_000398619.1 NZ_CP101274:280827-281123 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References