Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4327221..4327718 Replicon chromosome
Accession NZ_CP101269
Organism Escherichia coli strain EC21Z-048

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NML18_RS21115 Protein ID WP_001173343.1
Coordinates 4327221..4327394 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NML18_RS21120 Protein ID WP_000398619.1
Coordinates 4327422..4327718 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML18_RS21105 (4323891) 4323891..4325348 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
NML18_RS21110 (4325585) 4325585..4327102 + 1518 WP_001295087.1 lysine--tRNA ligase -
NML18_RS21115 (4327221) 4327221..4327394 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NML18_RS21120 (4327422) 4327422..4327718 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NML18_RS21125 (4327945) 4327945..4328217 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NML18_RS21130 (4328229) 4328229..4328459 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
NML18_RS21135 (4328640) 4328640..4330271 + 1632 WP_001216487.1 sensor histidine kinase -
NML18_RS21140 (4330268) 4330268..4330987 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T251746 WP_001173343.1 NZ_CP101269:c4327394-4327221 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT251746 WP_000398619.1 NZ_CP101269:c4327718-4327422 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References