Detailed information of TA system TA251743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4109355..4111038
• Replicon: chromosome
• Accession: NZ_CP101269
• Organism: Escherichia coli strain EC21Z-048

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: NML18_RS20000
• Protein ID: WP_000361918.1
• Coordinates: 4110121..4111038 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: NML18_RS19995
• Protein ID: WP_029488485.1
• Coordinates: 4109355..4110128 (+)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NML18_RS19980 (4105223)	4105223..4107307	+	2085	WP_001193075.1	protease Lon-related BREX system protein BrxL	-
NML18_RS19985 (4107426)	4107426..4108775	+	1350	WP_000766444.1	phospholipase D-like domain-containing protein	-
NML18_RS19990 (4108951)	4108951..4109271	-	321	Protein_3911	IS3 family transposase	-
NML18_RS19995 (4109355)	4109355..4110128	+	774	WP_029488485.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
NML18_RS20000 (4110121)	4110121..4111038	+	918	WP_000361918.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
NML18_RS20005 (4111332)	4111332..4111499	+	168	WP_001356018.1	hypothetical protein	-
NML18_RS20010 (4111552)	4111552..4112214	-	663	WP_104725624.1	hypothetical protein	-
NML18_RS20015 (4112287)	4112287..4112928	-	642	Protein_3916	integrase arm-type DNA-binding domain-containing protein	-
NML18_RS20020 (4113395)	4113395..4113538	-	144	Protein_3917	hypothetical protein	-
NML18_RS20025 (4113623)	4113623..4113820	-	198	WP_001327226.1	DUF957 domain-containing protein	-
NML18_RS20030 (4113840)	4113840..4114328	-	489	WP_000761685.1	DUF5983 family protein	-
NML18_RS20035 (4114325)	4114325..4114702	-	378	WP_001094443.1	TA system toxin CbtA family protein	-
NML18_RS20040 (4114792)	4114792..4115160	-	369	WP_001285607.1	type IV toxin-antitoxin system YeeU family antitoxin	-
NML18_RS20045 (4115240)	4115240..4115461	-	222	WP_000692350.1	DUF987 domain-containing protein	-
NML18_RS20050 (4115548)	4115548..4116024	-	477	WP_001186165.1	RadC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	tet(B)	-	4107426..4165677	58251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34962.10 Da
• Isoelectric Point: 4.9206

>T251743 WP_000361918.1 NZ_CP101269:4110121-4111038 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELKAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29315.73 Da
• Isoelectric Point: 9.9889

>AT251743 WP_029488485.1 NZ_CP101269:4109355-4110128 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPAVEWLIVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp