Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3564341..3565178 Replicon chromosome
Accession NZ_CP101269
Organism Escherichia coli strain EC21Z-048

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag NML18_RS17460 Protein ID WP_000227784.1
Coordinates 3564636..3565178 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag NML18_RS17455 Protein ID WP_001297137.1
Coordinates 3564341..3564652 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML18_RS17430 (3559361) 3559361..3560308 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
NML18_RS17435 (3560330) 3560330..3562321 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
NML18_RS17440 (3562311) 3562311..3562925 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
NML18_RS17445 (3562925) 3562925..3563254 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
NML18_RS17450 (3563266) 3563266..3564156 + 891 WP_000971336.1 heme o synthase -
NML18_RS17455 (3564341) 3564341..3564652 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
NML18_RS17460 (3564636) 3564636..3565178 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
NML18_RS17465 (3565234) 3565234..3566169 - 936 WP_254837452.1 tetratricopeptide repeat protein -
NML18_RS17470 (3566577) 3566577..3567941 + 1365 WP_001000960.1 MFS transporter -
NML18_RS17475 (3568069) 3568069..3568560 - 492 WP_001138904.1 nucleotide binding protein YajQ -
NML18_RS17480 (3568728) 3568728..3569639 + 912 WP_104725566.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T251741 WP_000227784.1 NZ_CP101269:3564636-3565178 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT251741 WP_001297137.1 NZ_CP101269:3564341-3564652 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References