Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 859102..859756 Replicon chromosome
Accession NZ_CP101269
Organism Escherichia coli strain EC21Z-048

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag NML18_RS04200 Protein ID WP_000244772.1
Coordinates 859349..859756 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NML18_RS04195 Protein ID WP_000354046.1
Coordinates 859102..859368 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML18_RS04170 (854390) 854390..855133 + 744 WP_001436959.1 SDR family oxidoreductase -
NML18_RS04175 (855190) 855190..856623 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
NML18_RS04180 (856668) 856668..856979 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
NML18_RS04185 (857143) 857143..857802 + 660 WP_000250274.1 hemolysin III family protein -
NML18_RS04190 (857879) 857879..858859 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
NML18_RS04195 (859102) 859102..859368 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NML18_RS04200 (859349) 859349..859756 + 408 WP_000244772.1 protein YgfX Toxin
NML18_RS04205 (859796) 859796..860317 - 522 WP_001055874.1 flavodoxin FldB -
NML18_RS04210 (860429) 860429..861325 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
NML18_RS04215 (861350) 861350..862060 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NML18_RS04220 (862066) 862066..863799 + 1734 WP_000813213.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T251731 WP_000244772.1 NZ_CP101269:859349-859756 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT251731 WP_000354046.1 NZ_CP101269:859102-859368 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References