Detailed information of TA system TA025173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 608278..608896
• Replicon: chromosome
• Accession: NC_015315
• Organism: Thermoproteus uzoniensis 768-20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F2L4L7
• Locus tag: TUZN_RS03375
• Protein ID: WP_013679531.1
• Coordinates: 608498..608896 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F2L4L6
• Locus tag: TUZN_RS03370
• Protein ID: WP_013679530.1
• Coordinates: 608278..608514 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TUZN_RS03345 (TUZN_0698)	603662..604813	+	1152	WP_013679525.1	cobalamin-independent methionine synthase II family protein	-
TUZN_RS03350 (TUZN_0699)	604917..605396	+	480	WP_013679526.1	PaREP1 family protein	-
TUZN_RS11425 (TUZN_0700)	605632..606903	-	1272	WP_013679527.1	hypothetical protein	-
TUZN_RS03360 (TUZN_0701)	606916..607506	-	591	WP_013679528.1	MarC family protein	-
TUZN_RS11430 (TUZN_0703)	607839..608015	-	177	WP_013679529.1	chromatin protein Cren7	-
TUZN_RS03370 (TUZN_0704)	608278..608514	+	237	WP_013679530.1	hypothetical protein	Antitoxin
TUZN_RS03375 (TUZN_0705)	608498..608896	+	399	WP_013679531.1	type II toxin-antitoxin system VapC family toxin	Toxin
TUZN_RS03380 (TUZN_0706)	609144..610256	-	1113	WP_013679532.1	cytochrome ubiquinol oxidase subunit I	-
TUZN_RS03385 (TUZN_0707)	610249..611892	-	1644	WP_013679533.1	cytochrome ubiquinol oxidase subunit I	-
TUZN_RS03390 (TUZN_0708)	612055..612555	+	501	WP_013679534.1	helix-turn-helix domain-containing protein	-
TUZN_RS03395 (TUZN_0709)	612585..613088	+	504	WP_013679535.1	metallophosphoesterase	-
TUZN_RS03400 (TUZN_0710)	613085..613573	+	489	WP_013679536.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-123)

Antitoxin

• Putative antitoxin VapB3-like (2-74)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14814.21 Da
• Isoelectric Point: 4.5727

>T25173 WP_013679531.1 NC_015315:608498-608896 [Thermoproteus uzoniensis 768-20]
MEIAADASAIAALFLPEDKSAVIEKLFGQAEAIHTLDLAAYEVLNAVWKYARRGVIGRRLAEEIETELLDLLRTLYIHRY
EEVLPDALKLALEKDVTVYDAAYVALAQKLGAPLLTLDRRLAEEFPRLAYMP

Nucleotide

• Length: 399 bp

>T25173 NC_015315:608498-608896 [Thermoproteus uzoniensis 768-20]
ATGGAGATAGCCGCTGATGCCTCGGCTATCGCCGCCCTCTTCTTGCCGGAGGACAAAAGCGCCGTCATTGAGAAGCTGTT
CGGGCAGGCTGAGGCTATACATACGCTGGATCTAGCCGCCTACGAGGTGCTGAACGCAGTGTGGAAATACGCCAGGAGAG
GCGTTATAGGCAGGAGGCTAGCCGAGGAGATTGAGACGGAGCTTCTGGATCTGCTCAGGACCTTGTACATCCACCGCTAC
GAGGAGGTGCTTCCCGACGCCCTCAAGTTAGCCTTAGAAAAAGACGTCACTGTATACGACGCCGCATATGTGGCTCTTGC
CCAGAAGCTGGGCGCGCCTCTGTTGACGTTAGACAGGAGACTGGCCGAGGAGTTCCCCCGCCTGGCCTACATGCCCTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9222.45 Da
• Isoelectric Point: 8.7675

>AT25173 WP_013679530.1 NC_015315:608278-608514 [Thermoproteus uzoniensis 768-20]
VSEVISIRVRRELKRALEELGIDYAEEVRRFLEQLVARERRRRALERARSLREAVEREAGALPHVAELIREDRDGDSR

Nucleotide

• Length: 237 bp

>AT25173 NC_015315:608278-608514 [Thermoproteus uzoniensis 768-20]
GTGAGCGAGGTTATCTCGATAAGGGTTAGAAGAGAGTTGAAAAGGGCGTTGGAGGAGCTGGGTATAGACTACGCCGAGGA
GGTCAGGAGGTTTCTGGAGCAACTTGTGGCTAGAGAGCGGAGGCGGAGGGCTCTGGAGAGGGCGCGGTCGCTGAGGGAAG
CTGTCGAGAGGGAGGCGGGCGCGTTGCCGCATGTCGCGGAGCTGATAAGGGAGGATAGAGATGGAGATAGCCGCTGA

Structures

Toxin

• AlphaFold DB: F2L4L7

Antitoxin

• AlphaFold DB: F2L4L6