Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 292531..293331 Replicon chromosome
Accession NZ_CP101269
Organism Escherichia coli strain EC21Z-048

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag NML18_RS01365 Protein ID WP_000342449.1
Coordinates 292804..293331 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NML18_RS01360 Protein ID WP_001277108.1
Coordinates 292531..292797 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML18_RS01340 (288189) 288189..288857 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
NML18_RS01345 (288850) 288850..289908 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
NML18_RS01350 (290153) 290153..291007 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
NML18_RS01355 (291278) 291278..292381 + 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NML18_RS01360 (292531) 292531..292797 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NML18_RS01365 (292804) 292804..293331 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NML18_RS01370 (293328) 293328..293711 - 384 WP_000778781.1 aspartate 1-decarboxylase autocleavage activator PanM -
NML18_RS01375 (294135) 294135..295244 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NML18_RS01380 (295292) 295292..296218 + 927 WP_000003004.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NML18_RS01385 (296215) 296215..297492 + 1278 WP_000803799.1 branched chain amino acid ABC transporter permease LivM -
NML18_RS01390 (297489) 297489..298256 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T251729 WP_000342449.1 NZ_CP101269:292804-293331 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT251729 WP_001277108.1 NZ_CP101269:292531-292797 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References