251725

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 33759..34285 Replicon plasmid pEC21Z063-100K
Accession NZ_CP101265
Organism Escherichia coli strain EC21Z-063

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag NML19_RS23640 Protein ID WP_000323025.1
Coordinates 33998..34285 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag NML19_RS23635 Protein ID WP_000534858.1
Coordinates 33759..33998 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML19_RS23595 (NML19_23590) 29433..30130 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
NML19_RS23600 (NML19_23595) 30457..30829 + 373 Protein_23 IS1 family transposase -
NML19_RS23605 (NML19_23600) 30843..31451 + 609 Protein_24 DDE-type integrase/transposase/recombinase -
NML19_RS23610 (NML19_23605) 31448..32229 + 782 Protein_25 IS21-like element helper ATPase IstB -
NML19_RS23615 (NML19_23610) 32321..32443 - 123 Protein_26 IS66 family insertion sequence element accessory protein TnpB -
NML19_RS23620 (NML19_23615) 32507..33211 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
NML19_RS23625 (NML19_23620) 33268..33417 - 150 WP_000550473.1 hypothetical protein -
NML19_RS23630 (NML19_23625) 33624..33734 - 111 Protein_29 hypothetical protein -
NML19_RS23635 (NML19_23630) 33759..33998 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
NML19_RS23640 (NML19_23635) 33998..34285 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
NML19_RS23645 (NML19_23640) 34568..34792 + 225 Protein_32 hypothetical protein -
NML19_RS23650 (NML19_23645) 35229..35438 + 210 WP_072662162.1 hypothetical protein -
NML19_RS23655 (NML19_23650) 35444..35933 - 490 Protein_34 IS3 family transposase -
NML19_RS23660 (NML19_23655) 36014..37522 - 1509 WP_141223309.1 group II intron reverse transcriptase/maturase -
NML19_RS23665 (NML19_23660) 38195..38620 - 426 Protein_36 IS3 family transposase -
NML19_RS23670 (NML19_23665) 38608..38688 + 81 Protein_37 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid cmlA1 / blaOXA-10 / ant(3'')-Ia / dfrA14 - 1..100090 100090


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T251725 WP_000323025.1 NZ_CP101265:33998-34285 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT251725 WP_000534858.1 NZ_CP101265:33759-33998 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References