Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 33759..34285 Replicon plasmid pEC21Z063-100K
Accession NZ_CP101265
Organism Escherichia coli strain EC21Z-063

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag NML19_RS23640 Protein ID WP_000323025.1
Coordinates 33998..34285 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag NML19_RS23635 Protein ID WP_000534858.1
Coordinates 33759..33998 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML19_RS23595 (NML19_23590) 29433..30130 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
NML19_RS23600 (NML19_23595) 30457..30829 + 373 Protein_23 IS1 family transposase -
NML19_RS23605 (NML19_23600) 30843..31451 + 609 Protein_24 DDE-type integrase/transposase/recombinase -
NML19_RS23610 (NML19_23605) 31448..32229 + 782 Protein_25 IS21-like element helper ATPase IstB -
NML19_RS23615 (NML19_23610) 32321..32443 - 123 Protein_26 IS66 family insertion sequence element accessory protein TnpB -
NML19_RS23620 (NML19_23615) 32507..33211 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
NML19_RS23625 (NML19_23620) 33268..33417 - 150 WP_000550473.1 hypothetical protein -
NML19_RS23630 (NML19_23625) 33624..33734 - 111 Protein_29 hypothetical protein -
NML19_RS23635 (NML19_23630) 33759..33998 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
NML19_RS23640 (NML19_23635) 33998..34285 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
NML19_RS23645 (NML19_23640) 34568..34792 + 225 Protein_32 hypothetical protein -
NML19_RS23650 (NML19_23645) 35229..35438 + 210 WP_072662162.1 hypothetical protein -
NML19_RS23655 (NML19_23650) 35444..35933 - 490 Protein_34 IS3 family transposase -
NML19_RS23660 (NML19_23655) 36014..37522 - 1509 WP_141223309.1 group II intron reverse transcriptase/maturase -
NML19_RS23665 (NML19_23660) 38195..38620 - 426 Protein_36 IS3 family transposase -
NML19_RS23670 (NML19_23665) 38608..38688 + 81 Protein_37 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid cmlA1 / blaOXA-10 / ant(3'')-Ia / dfrA14 - 1..100090 100090


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T251725 WP_000323025.1 NZ_CP101265:33998-34285 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT251725 WP_000534858.1 NZ_CP101265:33759-33998 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References