Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 83961..85584 Replicon plasmid pEC21Z063-110K-NDM5
Accession NZ_CP101264
Organism Escherichia coli strain EC21Z-063

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag NML19_RS23330 Protein ID WP_000088046.1
Coordinates 84259..85584 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag NML19_RS23325 Protein ID WP_024136327.1
Coordinates 83961..84254 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML19_RS23300 (NML19_23295) 79833..81074 + 1242 WP_001053338.1 VWA domain-containing protein -
NML19_RS23305 (NML19_23300) 81637..81918 - 282 WP_000077926.1 hypothetical protein -
NML19_RS23310 (NML19_23305) 81968..82159 - 192 WP_000398480.1 hypothetical protein -
NML19_RS23315 (NML19_23310) 82251..82592 - 342 WP_001151575.1 hypothetical protein -
NML19_RS23320 (NML19_23315) 82965..83357 + 393 WP_000341066.1 hypothetical protein -
NML19_RS23325 (NML19_23320) 83961..84254 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
NML19_RS23330 (NML19_23325) 84259..85584 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
NML19_RS23335 (NML19_23330) 85645..85851 + 207 WP_000134171.1 hypothetical protein -
NML19_RS23340 (NML19_23335) 85953..86363 + 411 WP_000985911.1 hypothetical protein -
NML19_RS23345 (NML19_23340) 86376..87191 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
NML19_RS23350 (NML19_23345) 87445..87870 + 426 WP_001043843.1 hypothetical protein -
NML19_RS23355 (NML19_23350) 88419..88727 + 309 WP_001224687.1 hypothetical protein -
NML19_RS23360 (NML19_23355) 88743..89600 + 858 WP_000784387.1 HNH endonuclease signature motif containing protein -
NML19_RS23365 (NML19_23360) 89662..89865 + 204 WP_001194555.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-5 / mph(A) / erm(42) - 1..109419 109419


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T251724 WP_000088046.1 NZ_CP101264:84259-85584 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT251724 WP_024136327.1 NZ_CP101264:83961-84254 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References