Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4046181..4046757 Replicon chromosome
Accession NZ_CP101263
Organism Escherichia coli strain EC21Z-063

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag NML19_RS19765 Protein ID WP_001299718.1
Coordinates 4046470..4046757 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0E2KXJ8
Locus tag NML19_RS19760 Protein ID WP_000063159.1
Coordinates 4046181..4046483 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NML19_RS19740 (4041865) 4041865..4042377 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
NML19_RS19745 (4042770) 4042770..4044920 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
NML19_RS19750 (4044970) 4044970..4045173 + 204 WP_000467859.1 YbdD/YjiX family protein -
NML19_RS19755 (4045184) 4045184..4046140 + 957 WP_001299717.1 GTPase -
NML19_RS19760 (4046181) 4046181..4046483 - 303 WP_000063159.1 BrnA antitoxin family protein Antitoxin
NML19_RS19765 (4046470) 4046470..4046757 - 288 WP_001299718.1 BrnT family toxin Toxin
NML19_RS19770 (4046954) 4046954..4048120 + 1167 Protein_3861 restriction endonuclease -
NML19_RS19775 (4048184) 4048184..4049803 + 1620 WP_001029740.1 class I SAM-dependent DNA methyltransferase -
NML19_RS19780 (4049793) 4049793..4050581 + 789 Protein_3863 restriction endonuclease subunit S -
NML19_RS19785 (4050636) 4050636..4051333 + 698 WP_103215986.1 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T251721 WP_001299718.1 NZ_CP101263:c4046757-4046470 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11536.01 Da        Isoelectric Point: 9.4646

>AT251721 WP_000063159.1 NZ_CP101263:c4046483-4046181 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEEIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2KXJ8

References