Detailed information of TA system TA025172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 174046..174661
• Replicon: chromosome
• Accession: NC_015315
• Organism: Thermoproteus uzoniensis 768-20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F2L1U5
• Locus tag: TUZN_RS00860
• Protein ID: WP_013679022.1
• Coordinates: 174046..174444 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F2L1U6
• Locus tag: TUZN_RS00865
• Protein ID: WP_013679023.1
• Coordinates: 174434..174661 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TUZN_RS00835 (TUZN_0181)	169207..170115	+	909	WP_013679017.1	dihydrodipicolinate synthase family protein	-
TUZN_RS00840 (TUZN_0182)	170158..170430	+	273	WP_013679018.1	ACT domain-containing protein	-
TUZN_RS00845 (TUZN_0183)	170427..171776	+	1350	WP_013679019.1	PFL family protein	-
TUZN_RS00850 (TUZN_0184)	171854..172894	+	1041	WP_013679020.1	formate--phosphoribosylaminoimidazolecarboxamide ligase family protein	-
TUZN_RS00855 (TUZN_0185)	172894..173895	+	1002	WP_148678661.1	formate--phosphoribosylaminoimidazolecarboxamide ligase	-
TUZN_RS00860 (TUZN_0186)	174046..174444	-	399	WP_013679022.1	type II toxin-antitoxin system VapC family toxin	Toxin
TUZN_RS00865 (TUZN_0187)	174434..174661	-	228	WP_013679023.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
TUZN_RS00870 (TUZN_0188)	174764..175477	+	714	WP_013679024.1	ribonuclease Z	-
TUZN_RS00875 (TUZN_0189)	175456..175983	+	528	WP_148678541.1	DUF359 domain-containing protein	-
TUZN_RS11310 (TUZN_0190)	175980..176135	-	156	WP_013679026.1	hypothetical protein	-
TUZN_RS00880 (TUZN_0191)	176197..177012	-	816	WP_013679027.1	MBL fold metallo-hydrolase	-
TUZN_RS00885 (TUZN_0192)	177040..178281	-	1242	WP_013679028.1	tRNA(Ile)(2)-agmatinylcytidine synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Post-segregation antitoxin CcdA (6-63)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15027.27 Da
• Isoelectric Point: 7.1994

>T25172 WP_013679022.1 NC_015315:c174444-174046 [Thermoproteus uzoniensis 768-20]
MSVEYLLDASALYGLATHYDRWLKYRERLAILHLTIYEVGNALWKEARAGRLQWQRAARPLSRVISGLRVLDDPPLEEVL
RLAVERDLTFYDASYAYVAETLGLSLVTQDGELLRKSRAAIDVATFLSRLSA

Nucleotide

• Length: 399 bp

>T25172 NC_015315:c174444-174046 [Thermoproteus uzoniensis 768-20]
ATGAGCGTTGAGTATCTGCTCGACGCCTCTGCTCTCTACGGCTTGGCTACACACTACGACAGATGGCTGAAATATAGGGA
GAGGCTCGCGATACTCCACTTGACAATATACGAGGTCGGCAACGCCCTATGGAAGGAGGCGAGGGCGGGGAGACTGCAGT
GGCAGAGAGCCGCGAGGCCTCTCTCCCGCGTGATCTCCGGCTTGAGGGTGCTGGACGATCCTCCCCTGGAGGAGGTTCTG
AGGCTGGCTGTCGAGAGGGATCTGACGTTCTACGACGCGAGCTACGCATACGTCGCGGAGACGCTGGGCTTGTCGTTGGT
GACGCAGGACGGCGAGTTGTTGCGCAAGTCGCGCGCCGCGATAGACGTGGCCACGTTCCTCTCGAGACTGTCGGCGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8890.14 Da
• Isoelectric Point: 5.0448

>AT25172 WP_013679023.1 NC_015315:c174661-174434 [Thermoproteus uzoniensis 768-20]
MSTTVISVRVRKELKERARELGIDIREVLEKALEEAIRRREEEELAKALEELKEALSRITEEEWVRAVRGSRDER

Nucleotide

• Length: 228 bp

>AT25172 NC_015315:c174661-174434 [Thermoproteus uzoniensis 768-20]
ATGTCGACTACGGTAATAAGCGTGAGGGTCAGGAAAGAGCTGAAGGAGAGGGCGAGGGAGCTCGGCATAGATATCAGGGA
GGTCTTAGAGAAGGCGCTTGAGGAGGCCATAAGGAGGAGGGAAGAGGAGGAGCTGGCCAAGGCGCTTGAGGAGTTGAAAG
AGGCCTTGTCGCGAATAACCGAGGAGGAGTGGGTGAGGGCAGTGCGCGGCTCTAGGGATGAGCGTTGA

Structures

Toxin

• AlphaFold DB: F2L1U5

Antitoxin

• AlphaFold DB: F2L1U6