Detailed information of TA system TA025171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 54186..54795
• Replicon: chromosome
• Accession: NC_015315
• Organism: Thermoproteus uzoniensis 768-20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: F2L112
• Locus tag: TUZN_RS00250
• Protein ID: WP_013678897.1
• Coordinates: 54400..54795 (+)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F2L111
• Locus tag: TUZN_RS00245
• Protein ID: WP_013678896.1
• Coordinates: 54186..54407 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TUZN_RS00230 (TUZN_0051)	51143..52147	-	1005	WP_052885971.1	polysulfide reductase NrfD	-
TUZN_RS00235 (TUZN_0052)	52214..52960	-	747	WP_052885972.1	hypothetical protein	-
TUZN_RS00240 (TUZN_0054)	53192..54142	+	951	WP_013678895.1	MBL fold metallo-hydrolase	-
TUZN_RS00245 (TUZN_0055)	54186..54407	+	222	WP_013678896.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
TUZN_RS00250 (TUZN_0056)	54400..54795	+	396	WP_013678897.1	PIN domain-containing protein	Toxin
TUZN_RS00255 (TUZN_0057)	55199..57283	-	2085	WP_013678898.1	GH116 family glycosyl hydrolase	-
TUZN_RS00260 (TUZN_0058)	57374..57928	+	555	WP_013678899.1	PaREP1 family protein	-
TUZN_RS00270 (TUZN_0060)	58456..59037	+	582	WP_148678528.1	hypothetical protein	-
TUZN_RS00275 (TUZN_0061)	59012..59743	-	732	WP_013678902.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-118)

Antitoxin

• SpoVT-AbrB domain (4-44)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14423.75 Da
• Isoelectric Point: 4.9344

>T25171 WP_013678897.1 NC_015315:54400-54795 [Thermoproteus uzoniensis 768-20]
MGEAVVDTNAVIYYVVEDSPFHGEAEALLDALDFWHLPTVVVHELVWFFKKAAPDRGIEVLRALLGYEKVVVECEDLASL
RKAVGASLAYYNDLVVILTARRLGLPLATFDKRMAKRAEALGVPVLGPRGD

Nucleotide

• Length: 396 bp

>T25171 NC_015315:54400-54795 [Thermoproteus uzoniensis 768-20]
ATGGGTGAGGCGGTCGTCGACACGAACGCGGTGATATACTACGTAGTCGAGGATTCGCCGTTTCATGGGGAGGCCGAGGC
GCTCCTCGACGCCCTAGACTTCTGGCACCTCCCCACCGTCGTCGTGCACGAACTAGTGTGGTTTTTCAAGAAGGCGGCGC
CGGATAGGGGGATCGAGGTCTTGAGGGCTCTCCTGGGCTACGAGAAGGTCGTCGTGGAGTGCGAGGACTTGGCGTCGTTG
AGAAAGGCGGTCGGCGCGAGTCTCGCCTACTACAACGACCTAGTAGTTATCTTGACGGCGAGGAGGCTCGGCCTTCCCCT
GGCAACCTTCGACAAGAGGATGGCGAAGAGGGCCGAGGCTCTTGGGGTTCCGGTGCTCGGGCCTCGGGGCGATTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8018.43 Da
• Isoelectric Point: 10.4418

>AT25171 WP_013678896.1 NC_015315:54186-54407 [Thermoproteus uzoniensis 768-20]
MAVVKVTRNFQITIPADVRKALGIREGDRLLVEVEGDRIVIRKAAGALPRIRLGMRLAPEDIDRVIEQGVADG

Nucleotide

• Length: 222 bp

>AT25171 NC_015315:54186-54407 [Thermoproteus uzoniensis 768-20]
ATGGCGGTGGTCAAGGTGACGCGCAACTTCCAGATAACGATCCCGGCGGATGTCAGGAAGGCGTTGGGCATCCGCGAGGG
GGACCGCCTGCTGGTGGAGGTCGAGGGCGACCGGATAGTGATAAGGAAGGCGGCGGGCGCGTTGCCGAGGATTAGGCTGG
GGATGAGGCTGGCGCCGGAGGACATAGACAGAGTTATAGAACAAGGCGTAGCCGATGGGTGA

Structures

Toxin

• AlphaFold DB: F2L112

Antitoxin

• AlphaFold DB: F2L111